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Imaging of Human Blood Cells #77

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opened 2025-06-15 00:47:32 +00:00 by hugo · 4 comments
hugo commented 2025-06-15 00:47:32 +00:00
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A detailed protocol for sample preparation can be found at https://www.leica-microsystems.com/science-lab/life-science/human-blood-cells-protocol.

  1. Sample Collection
  2. Cell Separation
  3. Fixation and Dehydration
  4. Post-Fixation

Discussion on Post-Fixation of Cells:

Some Information about the use of OsO4 for Dihydroxylation https://www.masterorganicchemistry.com/2011/07/01/reagent-friday-oso4-osmium-tetroxide/.

A detailed protocol for sample preparation can be found at https://www.leica-microsystems.com/science-lab/life-science/human-blood-cells-protocol. 1. Sample Collection 2. Cell Separation 3. Fixation and Dehydration 4. Post-Fixation Discussion on Post-Fixation of Cells: - https://www.researchgate.net/post/Is_Osmium_tetroxide_necessary_for_SEM - https://www.researchgate.net/post/Instead-of-osmium-tetroxide-is-there-any-other-substance-that-can-be-used-as-a-secondary-fixative-for-electron-microscopy-of-bacterial-cell Some Information about the use of OsO4 for Dihydroxylation https://www.masterorganicchemistry.com/2011/07/01/reagent-friday-oso4-osmium-tetroxide/.
forgejo-actions commented 2025-06-15 00:47:38 +00:00
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Partial Techtree

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    0:::eoi
    0["<a href='https://git.fa-fo.de/fafo/techtree/issues/77' target='_blank'>#77</a> | MISSING\n<i>Process</i>\n<b>Imaging of Human Blood Cells</b>"]
    1:::dep_missing
    1["<a href='https://git.fa-fo.de/fafo/techtree/issues/38' target='_blank'>#38</a> | MISSING\n<i>Process</i>\n<b>SEM Non-Metallic Imaging</b>"]
    2:::dep_missing
    2["<a href='https://git.fa-fo.de/fafo/techtree/issues/57' target='_blank'>#57</a> | MISSING\n<i>Process</i>\n<b>Metal thin film deposition</b>"]
    3:::dep_missing
    3["<a href='https://git.fa-fo.de/fafo/techtree/issues/56' target='_blank'>#56</a> | MISSING\n<i>Process</i>\n<b>PVD: Thermal Evaporation</b>"]
    4:::dep_missing
    4["<a href='https://git.fa-fo.de/fafo/techtree/issues/37' target='_blank'>#37</a> | MISSING\n<i>Process</i>\n<b>PVD: Sputtering</b>"]
    5:::dep_completed
    5["<a href='https://git.fa-fo.de/fafo/techtree/issues/28' target='_blank'>#28</a> | COMPLETED\n<i>Equipment</i>\n<b>Scanning Electron Microscope (SEM)</b>"]
    6:::dep_completed
    6["<a href='https://git.fa-fo.de/fafo/techtree/issues/26' target='_blank'>#26</a> | COMPLETED\n<i>Equipment</i>\n<b>Water Cooling</b>"]
    7:::dep_completed
    7["<a href='https://git.fa-fo.de/fafo/techtree/issues/31' target='_blank'>#31</a> | COMPLETED\n<i>Process</i>\n<b>SEM Imaging@100nm</b>"]
    8:::dep_completed
    8["<a href='https://git.fa-fo.de/fafo/techtree/issues/30' target='_blank'>#30</a> | COMPLETED\n<i>Process</i>\n<b>SEM Imaging@1µm</b>"]
    9:::dep_completed
    9["<a href='https://git.fa-fo.de/fafo/techtree/issues/29' target='_blank'>#29</a> | COMPLETED\n<i>Process</i>\n<b>SEM Imaging@10µm</b>"]
    0 --> 7
    0 --> 1
    2 --> 4
    2 --> 3
    1 --> 5
    1 --> 2
    7 --> 8
    8 --> 9
    9 --> 5
    5 --> 6

Digest: 5f7fa97f244e2d1fd347e99d8846ab017409097de0f6da198958c1a5cdd3a182; Last Updated: 2025-06-16 16:24:02

## Partial Techtree ```mermaid flowchart BT classDef eoi fill:#fff, stroke:#000, color:#000; classDef dependant fill:#fff, stroke:#888, color:#888; classDef dep_missing fill:#fcc, stroke:#800, color:#000; classDef dep_assigned fill:#ffa, stroke:#a50, color:#000; classDef dep_completed fill:#afa, stroke:#080, color:#000; 0:::eoi 0["<a href='https://git.fa-fo.de/fafo/techtree/issues/77' target='_blank'>#77</a> | MISSING\n<i>Process</i>\n<b>Imaging of Human Blood Cells</b>"] 1:::dep_missing 1["<a href='https://git.fa-fo.de/fafo/techtree/issues/38' target='_blank'>#38</a> | MISSING\n<i>Process</i>\n<b>SEM Non-Metallic Imaging</b>"] 2:::dep_missing 2["<a href='https://git.fa-fo.de/fafo/techtree/issues/57' target='_blank'>#57</a> | MISSING\n<i>Process</i>\n<b>Metal thin film deposition</b>"] 3:::dep_missing 3["<a href='https://git.fa-fo.de/fafo/techtree/issues/56' target='_blank'>#56</a> | MISSING\n<i>Process</i>\n<b>PVD: Thermal Evaporation</b>"] 4:::dep_missing 4["<a href='https://git.fa-fo.de/fafo/techtree/issues/37' target='_blank'>#37</a> | MISSING\n<i>Process</i>\n<b>PVD: Sputtering</b>"] 5:::dep_completed 5["<a href='https://git.fa-fo.de/fafo/techtree/issues/28' target='_blank'>#28</a> | COMPLETED\n<i>Equipment</i>\n<b>Scanning Electron Microscope (SEM)</b>"] 6:::dep_completed 6["<a href='https://git.fa-fo.de/fafo/techtree/issues/26' target='_blank'>#26</a> | COMPLETED\n<i>Equipment</i>\n<b>Water Cooling</b>"] 7:::dep_completed 7["<a href='https://git.fa-fo.de/fafo/techtree/issues/31' target='_blank'>#31</a> | COMPLETED\n<i>Process</i>\n<b>SEM Imaging@100nm</b>"] 8:::dep_completed 8["<a href='https://git.fa-fo.de/fafo/techtree/issues/30' target='_blank'>#30</a> | COMPLETED\n<i>Process</i>\n<b>SEM Imaging@1µm</b>"] 9:::dep_completed 9["<a href='https://git.fa-fo.de/fafo/techtree/issues/29' target='_blank'>#29</a> | COMPLETED\n<i>Process</i>\n<b>SEM Imaging@10µm</b>"] 0 --> 7 0 --> 1 2 --> 4 2 --> 3 1 --> 5 1 --> 2 7 --> 8 8 --> 9 9 --> 5 5 --> 6 ``` <small>Digest: 5f7fa97f244e2d1fd347e99d8846ab017409097de0f6da198958c1a5cdd3a182; Last Updated: 2025-06-16 16:24:02</small>
hugo added the
Type
Process
 label 2025-06-15 00:49:02 +00:00
hugo added a new dependency 2025-06-15 00:53:44 +00:00
#68 Heating Stirrer
hugo removed a dependency 2025-06-15 00:53:52 +00:00
#68 Heating Stirrer
q3k commented 2025-06-15 00:57:08 +00:00
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Should depend on #4 but we're having issues with the dependencies dropdown.

Should depend on #4 but we're having issues with the dependencies dropdown.
rahix commented 2025-06-15 12:07:17 +00:00
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Instead of depending on #4 directly, please create (an) intermediate Process element which details what the Wet Lab is going to be used for in this application. You can group everything into one element, if the processes are not wildly unrelated.

Instead of depending on #4 directly, please create (an) intermediate _Process_ element which details what the Wet Lab is going to be used for in this application. You can group everything into one element, if the processes are not wildly unrelated.
rahix commented 2025-06-16 16:20:13 +00:00
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This will also need #57 (Metal Thin Film Deposition), right? Or more concretely, it specifically requires #37 (Sputtering)?

This will also need #57 (Metal Thin Film Deposition), right? Or more concretely, it specifically requires #37 (Sputtering)?
rahix added a new dependency 2025-06-16 16:22:15 +00:00
#31 SEM Imaging@100nm
rahix added a new dependency 2025-06-16 16:23:44 +00:00
#38 SEM Non-Metallic Imaging
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#31 SEM Imaging@100nm
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#38 SEM Non-Metallic Imaging
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Reference: fafo/techtree#77
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