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Imaging of Human Blood Cells #77

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opened 2025-06-15 00:47:32 +00:00 by hugo · 4 comments
hugo commented 2025-06-15 00:47:32 +00:00
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A detailed protocol for sample preparation can be found at https://www.leica-microsystems.com/science-lab/life-science/human-blood-cells-protocol.

  1. Sample Collection
  2. Cell Separation
  3. Fixation and Dehydration
  4. Post-Fixation

Discussion on Post-Fixation of Cells:

Some Information about the use of OsO4 for Dihydroxylation https://www.masterorganicchemistry.com/2011/07/01/reagent-friday-oso4-osmium-tetroxide/.

A detailed protocol for sample preparation can be found at https://www.leica-microsystems.com/science-lab/life-science/human-blood-cells-protocol. 1. Sample Collection 2. Cell Separation 3. Fixation and Dehydration 4. Post-Fixation Discussion on Post-Fixation of Cells: - https://www.researchgate.net/post/Is_Osmium_tetroxide_necessary_for_SEM - https://www.researchgate.net/post/Instead-of-osmium-tetroxide-is-there-any-other-substance-that-can-be-used-as-a-secondary-fixative-for-electron-microscopy-of-bacterial-cell Some Information about the use of OsO4 for Dihydroxylation https://www.masterorganicchemistry.com/2011/07/01/reagent-friday-oso4-osmium-tetroxide/.
forgejo-actions commented 2025-06-15 00:47:38 +00:00
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Partial Techtree

flowchart BT

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    0:::eoi
    0["<a href='https://git.fa-fo.de/fafo/techtree/issues/77' target='_blank'>#77</a> | MISSING<br/><i>Process</i><br/><b>Imaging of Human Blood Cells</b>"]
    1:::dep_missing
    1["<a href='https://git.fa-fo.de/fafo/techtree/issues/38' target='_blank'>#38</a> | MISSING<br/><i>Process</i><br/><b>SEM Non-Metallic Imaging</b>"]
    2:::dep_missing
    2["<a href='https://git.fa-fo.de/fafo/techtree/issues/57' target='_blank'>#57</a> | MISSING<br/><i>Process</i><br/><b>Metal thin film deposition</b>"]
    3:::dep_missing
    3["<a href='https://git.fa-fo.de/fafo/techtree/issues/56' target='_blank'>#56</a> | MISSING<br/><i>Process</i><br/><b>PVD: Thermal Evaporation</b>"]
    4:::dep_completed
    4["<a href='https://git.fa-fo.de/fafo/techtree/issues/121' target='_blank'>#121</a> | COMPLETED<br/><i>Equipment</i><br/><b>Thermal Evaporation Coater</b>"]
    5:::dep_missing
    5["<a href='https://git.fa-fo.de/fafo/techtree/issues/37' target='_blank'>#37</a> | MISSING<br/><i>Process</i><br/><b>PVD: Sputtering</b>"]
    6:::dep_missing
    6["<a href='https://git.fa-fo.de/fafo/techtree/issues/79' target='_blank'>#79</a> | MISSING<br/><i>Equipment</i><br/><b>Sputter Coater</b>"]
    7:::dep_completed
    7["<a href='https://git.fa-fo.de/fafo/techtree/issues/28' target='_blank'>#28</a> | COMPLETED<br/><i>Equipment</i><br/><b>Scanning Electron Microscope (SEM)</b>"]
    8:::dep_completed
    8["<a href='https://git.fa-fo.de/fafo/techtree/issues/26' target='_blank'>#26</a> | COMPLETED<br/><i>Equipment</i><br/><b>Water Cooling</b>"]
    9:::dep_completed
    9["<a href='https://git.fa-fo.de/fafo/techtree/issues/31' target='_blank'>#31</a> | COMPLETED<br/><i>Process</i><br/><b>SEM Imaging@100nm</b>"]
    10:::dep_completed
    10["<a href='https://git.fa-fo.de/fafo/techtree/issues/30' target='_blank'>#30</a> | COMPLETED<br/><i>Process</i><br/><b>SEM Imaging@1µm</b>"]
    11:::dep_completed
    11["<a href='https://git.fa-fo.de/fafo/techtree/issues/29' target='_blank'>#29</a> | COMPLETED<br/><i>Process</i><br/><b>SEM Imaging@10µm</b>"]
    12:::dep_assigned
    12["<a href='https://git.fa-fo.de/fafo/techtree/issues/4' target='_blank'>#4</a> | ASSIGNED<br/><i>Equipment</i><br/><b>Wet Lab (Chemistry)</b>"]
    13:::dep_assigned
    13["<a href='https://git.fa-fo.de/fafo/techtree/issues/118' target='_blank'>#118</a> | ASSIGNED<br/><i>Development</i><br/><b>Hazard Assessment</b>"]
    14:::dep_completed
    14["<a href='https://git.fa-fo.de/fafo/techtree/issues/68' target='_blank'>#68</a> | COMPLETED<br/><i>Equipment</i><br/><b>Heating Stirrer</b>"]
    15:::dep_assigned
    15["<a href='https://git.fa-fo.de/fafo/techtree/issues/67' target='_blank'>#67</a> | ASSIGNED<br/><i>Equipment</i><br/><b>Chemical Storage/Containment</b>"]
    16:::dep_assigned
    16["<a href='https://git.fa-fo.de/fafo/techtree/issues/27' target='_blank'>#27</a> | ASSIGNED<br/><i>Equipment</i><br/><b>Technical Ventilation</b>"]
    17:::dep_assigned
    17["<a href='https://git.fa-fo.de/fafo/techtree/issues/23' target='_blank'>#23</a> | ASSIGNED<br/><i>Equipment</i><br/><b>Fume Hood</b>"]
    18:::dependant
    18["<a href='https://git.fa-fo.de/fafo/techtree/issues/112' target='_blank'>#112</a> | ULTIMATE GOAL<br/><i>Process</i><br/><b>Nanoscale Imaging</b>"]
    0 --> 12
    0 --> 9
    0 --> 1
    15 --> 16
    15 --> 13
    2 --> 5
    2 --> 3
    3 --> 4
    1 --> 7
    1 --> 2
    5 --> 6
    9 --> 10
    10 --> 11
    11 --> 7
    7 --> 8
    16 --> 13
    17 --> 16
    12 --> 17
    12 --> 16
    12 --> 15
    12 --> 14
    12 --> 13
    18 --> 0

Digest: 489fbcd1f4af9d71a40220ec7a8de2975a9e976622b7b73e23bb2fd21203cb4c; Last Updated: 2026-04-20 10:20:26

## Partial Techtree ```mermaid flowchart BT classDef eoi fill:#fff, stroke:#000, color:#000; classDef dependant fill:#fff, stroke:#888, color:#888; classDef ultimate fill:#fff, stroke:#000, color:#000; classDef dep_missing fill:#fcc, stroke:#800, color:#000; classDef dep_assigned fill:#ffa, stroke:#a50, color:#000; classDef dep_completed fill:#afa, stroke:#080, color:#000; 0:::eoi 0["<a href='https://git.fa-fo.de/fafo/techtree/issues/77' target='_blank'>#77</a> | MISSING<br/><i>Process</i><br/><b>Imaging of Human Blood Cells</b>"] 1:::dep_missing 1["<a href='https://git.fa-fo.de/fafo/techtree/issues/38' target='_blank'>#38</a> | MISSING<br/><i>Process</i><br/><b>SEM Non-Metallic Imaging</b>"] 2:::dep_missing 2["<a href='https://git.fa-fo.de/fafo/techtree/issues/57' target='_blank'>#57</a> | MISSING<br/><i>Process</i><br/><b>Metal thin film deposition</b>"] 3:::dep_missing 3["<a href='https://git.fa-fo.de/fafo/techtree/issues/56' target='_blank'>#56</a> | MISSING<br/><i>Process</i><br/><b>PVD: Thermal Evaporation</b>"] 4:::dep_completed 4["<a href='https://git.fa-fo.de/fafo/techtree/issues/121' target='_blank'>#121</a> | COMPLETED<br/><i>Equipment</i><br/><b>Thermal Evaporation Coater</b>"] 5:::dep_missing 5["<a href='https://git.fa-fo.de/fafo/techtree/issues/37' target='_blank'>#37</a> | MISSING<br/><i>Process</i><br/><b>PVD: Sputtering</b>"] 6:::dep_missing 6["<a href='https://git.fa-fo.de/fafo/techtree/issues/79' target='_blank'>#79</a> | MISSING<br/><i>Equipment</i><br/><b>Sputter Coater</b>"] 7:::dep_completed 7["<a href='https://git.fa-fo.de/fafo/techtree/issues/28' target='_blank'>#28</a> | COMPLETED<br/><i>Equipment</i><br/><b>Scanning Electron Microscope (SEM)</b>"] 8:::dep_completed 8["<a href='https://git.fa-fo.de/fafo/techtree/issues/26' target='_blank'>#26</a> | COMPLETED<br/><i>Equipment</i><br/><b>Water Cooling</b>"] 9:::dep_completed 9["<a href='https://git.fa-fo.de/fafo/techtree/issues/31' target='_blank'>#31</a> | COMPLETED<br/><i>Process</i><br/><b>SEM Imaging@100nm</b>"] 10:::dep_completed 10["<a href='https://git.fa-fo.de/fafo/techtree/issues/30' target='_blank'>#30</a> | COMPLETED<br/><i>Process</i><br/><b>SEM Imaging@1µm</b>"] 11:::dep_completed 11["<a href='https://git.fa-fo.de/fafo/techtree/issues/29' target='_blank'>#29</a> | COMPLETED<br/><i>Process</i><br/><b>SEM Imaging@10µm</b>"] 12:::dep_assigned 12["<a href='https://git.fa-fo.de/fafo/techtree/issues/4' target='_blank'>#4</a> | ASSIGNED<br/><i>Equipment</i><br/><b>Wet Lab (Chemistry)</b>"] 13:::dep_assigned 13["<a href='https://git.fa-fo.de/fafo/techtree/issues/118' target='_blank'>#118</a> | ASSIGNED<br/><i>Development</i><br/><b>Hazard Assessment</b>"] 14:::dep_completed 14["<a href='https://git.fa-fo.de/fafo/techtree/issues/68' target='_blank'>#68</a> | COMPLETED<br/><i>Equipment</i><br/><b>Heating Stirrer</b>"] 15:::dep_assigned 15["<a href='https://git.fa-fo.de/fafo/techtree/issues/67' target='_blank'>#67</a> | ASSIGNED<br/><i>Equipment</i><br/><b>Chemical Storage/Containment</b>"] 16:::dep_assigned 16["<a href='https://git.fa-fo.de/fafo/techtree/issues/27' target='_blank'>#27</a> | ASSIGNED<br/><i>Equipment</i><br/><b>Technical Ventilation</b>"] 17:::dep_assigned 17["<a href='https://git.fa-fo.de/fafo/techtree/issues/23' target='_blank'>#23</a> | ASSIGNED<br/><i>Equipment</i><br/><b>Fume Hood</b>"] 18:::dependant 18["<a href='https://git.fa-fo.de/fafo/techtree/issues/112' target='_blank'>#112</a> | ULTIMATE GOAL<br/><i>Process</i><br/><b>Nanoscale Imaging</b>"] 0 --> 12 0 --> 9 0 --> 1 15 --> 16 15 --> 13 2 --> 5 2 --> 3 3 --> 4 1 --> 7 1 --> 2 5 --> 6 9 --> 10 10 --> 11 11 --> 7 7 --> 8 16 --> 13 17 --> 16 12 --> 17 12 --> 16 12 --> 15 12 --> 14 12 --> 13 18 --> 0 ``` <small>Digest: 489fbcd1f4af9d71a40220ec7a8de2975a9e976622b7b73e23bb2fd21203cb4c; Last Updated: 2026-04-20 10:20:26</small>
hugo added the
Type
Process
 label 2025-06-15 00:49:02 +00:00
hugo added a new dependency 2025-06-15 00:53:44 +00:00
#68 Heating Stirrer
hugo removed a dependency 2025-06-15 00:53:52 +00:00
#68 Heating Stirrer
q3k commented 2025-06-15 00:57:08 +00:00
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Should depend on #4 but we're having issues with the dependencies dropdown.

Should depend on #4 but we're having issues with the dependencies dropdown.
rahix commented 2025-06-15 12:07:17 +00:00
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Instead of depending on #4 directly, please create (an) intermediate Process element which details what the Wet Lab is going to be used for in this application. You can group everything into one element, if the processes are not wildly unrelated.

Instead of depending on #4 directly, please create (an) intermediate _Process_ element which details what the Wet Lab is going to be used for in this application. You can group everything into one element, if the processes are not wildly unrelated.
rahix commented 2025-06-16 16:20:13 +00:00
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This will also need #57 (Metal Thin Film Deposition), right? Or more concretely, it specifically requires #37 (Sputtering)?

This will also need #57 (Metal Thin Film Deposition), right? Or more concretely, it specifically requires #37 (Sputtering)?
rahix added a new dependency 2025-06-16 16:22:15 +00:00
#31 SEM Imaging@100nm
rahix added a new dependency 2025-06-16 16:23:44 +00:00
#38 SEM Non-Metallic Imaging
rahix added a new dependency 2025-07-06 17:33:41 +00:00
#4 Wet Lab (Chemistry)
rahix added a new dependency 2025-11-09 00:59:16 +00:00
#112 Nanoscale Imaging
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#112 Nanoscale Imaging
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#4 Wet Lab (Chemistry)
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#31 SEM Imaging@100nm
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#38 SEM Non-Metallic Imaging
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Reference: fafo/techtree#77
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