openflexure-microscope-server/src/openflexure_microscope_server/background_detect.py
Julian Stirling 9b58f4d59e Apply suggestions from code review of branch scanning-stability
Co-authored-by: Joe Knapper <joe.knapper@glasgow.ac.uk>
2025-12-02 09:57:25 +00:00

373 lines
15 KiB
Python

"""Provide functionality to detect if the camera is imaging sample or background.
An example background image is captured by the camera and sent to classes in the module
for analysis. Information from these images is used to detect whether an image from the
current camera field of view contains sample.
"""
from typing import Optional, Any
import cv2
import numpy as np
from pydantic import BaseModel, Field, ConfigDict
from pydantic.errors import PydanticUserError
from labthings_fastapi.thing_description import type_to_dataschema
class MissingBackgroundDataError(RuntimeError):
"""An error raised if checking for sample without background data set."""
class ChannelBlankError(RuntimeError):
"""An error raised if a channel has no measured standard deviation.
This is not physical and usually means the camera has not yet booted or changed
mode fully.
"""
class BackgroundDetectorStatus(BaseModel):
"""The status information about a background detector instance needed for the GUI.
Each BackgroundDetectAlgorithm must be able to return one of these models when
``status`` is called.
"""
ready: bool
"""True if ready to be used, if False this detector isn't initialised for use.
This could be called ``has_background_data`` or similar, but the more generic
``ready`` is used in case more complex methods are added in the future, which
need different initialisation.
"""
settings: dict[str, Any]
"""The settings for the current background detect Algorithm. These are a dictionary
dumped from the base model."""
# Setting schema is a dict until LabThings FastAPI issue #154 is fixed and
# DataSchema can be used directly. For now `model_dump()` must be used to dump schema
# to a dict.
settings_schema: dict[str, Any]
"""The schema for the settings for the current background detect Algorithm.
This is reported so that the UI can dynamically create a UI for any background detector
algorithm.
"""
class BackgroundDetectAlgorithm:
"""The base class for defining background detect algorithms."""
background_data_model: BaseModel = BaseModel
"""The data model of the background data. This must be set by child classes"""
settings_data_model: BaseModel = BaseModel
"""The data model of algorithm settings. This must be set by child classes"""
def __init__(self) -> None:
"""Initialise the algorithm settings."""
try:
self._settings: BaseModel = self.settings_data_model()
except PydanticUserError as e:
raise NotImplementedError(
"BackgroundDetectAlgorithms must set their own settings data model."
) from e
@property
def status(self) -> BackgroundDetectorStatus:
"""The status information needed for the GUI. Read only."""
return BackgroundDetectorStatus(
ready=self.background_data is not None,
settings=self.settings.model_dump(),
# Dump model with `model_dump()` for reason explained when defining
# BackgroundDetectorStatus
settings_schema=type_to_dataschema(self.settings_data_model).model_dump(),
)
# Requires a getter and a setter to support being a BaseModel but being
# saved to file as a dict
_background_data: Optional[BaseModel] = None
@property
def background_data(self) -> Optional[BaseModel]:
"""The statistics of the background image."""
bd = self._background_data
if bd is None:
return None
return bd
@background_data.setter
def background_data(self, value: Optional[BaseModel | dict]) -> None:
"""Set the statistics for the background image.
This should be None, of no data is available. It can be set from either
a dictionary or a base model of the type specified in
``self.background_data_model``.
"""
try:
if value is None:
self._background_data = None
elif isinstance(value, self.background_data_model):
self._background_data = value
elif isinstance(value, dict):
self._background_data = self.background_data_model(**value)
else:
raise TypeError(
f"Cannot set background_data with an object of type {type(value)}"
)
except PydanticUserError as e:
raise NotImplementedError(
"BackgroundDetectAlgorithms must set their own background data model."
) from e
@property
def settings(self) -> BaseModel:
"""The statistics of the background image."""
return self._settings
@settings.setter
def settings(self, value: BaseModel | dict) -> None:
if isinstance(value, self.settings_data_model):
self._settings = value
elif isinstance(value, dict):
self._settings = self.settings_data_model(**value)
else:
raise TypeError(f"Cannot set settings with an object of type {type(value)}")
def image_is_sample(self, image: np.ndarray) -> tuple[bool, str]:
"""Label the current image as either background or sample.
:returns: A tuple of the result (boolean), and explanation string. The
explanation string is formatted so it can be added into a sentence such as
``An action was taken because the image is {message}.``
"""
raise NotImplementedError(
"Each background detect algorithm must implement an image_is_sample method."
)
def set_background(self, image: np.ndarray) -> None:
"""Use the input image to update the background data.
Background data must be a Pydantic BaseModel.
"""
raise NotImplementedError(
"Each background detect algorithm must implement an set_background method."
)
class ChannelDistributions(BaseModel):
"""A BaseModel for storing the channel distribution of a background image."""
means: list[float]
"""The mean of each channel in the colourspace."""
standard_deviations: list[float]
"""The standard deviation of each channel in the colourspace."""
class ColourChannelDetectSettings(BaseModel):
"""A BaseModel for storing the settings for colour channel detectors."""
model_config = ConfigDict(extra="forbid")
channel_tolerance: float = 7.0
"""Channel Tolerance
The number of standard deviations a pixel value must be from the background mean
to be considered sample.
"""
# Use Field to set Title reported to UI. By default Pydantic will convert the name
# from snake_case to Title Case.
min_sample_coverage: float = Field(25, title="Sample Coverage Required (%)")
"""Sample Coverage Required (%)
The minimum percentage of the image that needs to be identified as sample for the
image to be labeled as containing sample.
"""
class ColourChannelDetectLUV(BackgroundDetectAlgorithm):
"""Compare images with a known background in LUV colourspace.
This uses an LUV colour space checking only the mean and standard deviation of the
U and V channels. The LUV colourspace as it collect colours together in a human-
intuitive way.
"""
background_data_model: BaseModel = ChannelDistributions
settings_data_model: BaseModel = ColourChannelDetectSettings
# These are the same as those used for ChannelDeviationLUV. More detail is
# provided there.
min_stds = [0.5, 0.3, 0.5]
def background_mask(self, image: np.ndarray) -> np.ndarray:
"""Calculate a binary image, showing whether each pixel is background.
True is background.
The image should be in LUV format, the output will be binary with the
same shape in the first two dimensions.
"""
# The ``[1:]`` selects only the U and V channels of the image.
# Only U and V are used as brightness (L) often changes as
# the height of the sample changes.
# Wrapping in ``[[ ]]`` forces the colour channels to the numpy axis 2
# (3rd axis) so they are compared to the colour channel of each pixel.
means = np.array([[self.background_data.means[1:]]])
stds = np.array([[self.background_data.standard_deviations[1:]]])
# Compare each image in the pixel with the mean and standard deviation along
# axis to (the colour channels).
return np.all(
np.abs(image[:, :, 1:] - means) < stds * self.settings.channel_tolerance,
axis=2,
)
def get_sample_coverage(self, image: np.ndarray) -> float:
"""Return the percentage of the input image that is background.
Evaluate whether it is foreground or background by comparing it to the saved
statistics for a background image on a per-pixel basis
:returns: A value (between 0 and 100) is the percentage of the image that is
sample.
"""
if not self.background_data:
raise MissingBackgroundDataError(
"Background is not set: you need to calibrate background detection."
)
image_luv = cv2.cvtColor(image, cv2.COLOR_RGB2LUV)
mask = self.background_mask(image_luv)
return (1 - np.count_nonzero(mask) / np.prod(mask.shape)) * 100
def image_is_sample(self, image: np.ndarray) -> tuple[bool, str]:
"""Label the current image as either background or sample.
:returns: A tuple of the result (boolean), and explanation string. The
explanation string is formatted so it can be added into a sentence such as
``An action was taken because the image is {message}.``
"""
sample_coverage = self.get_sample_coverage(image)
# Use bool otherwise get numpy variants of True and False.
is_sample = bool(sample_coverage > self.settings.min_sample_coverage)
message = f"{sample_coverage:0.1f}% sample"
if not is_sample:
message = "only " + message
return is_sample, message
def set_background(self, image: np.ndarray) -> None:
"""Use the input image to update the background distributions."""
image_luv = cv2.cvtColor(image, cv2.COLOR_RGB2LUV)
mu = np.mean(image_luv, axis=(0, 1))
std = np.std(image_luv, axis=(0, 1))
if np.any(std == 0):
raise ChannelBlankError("Some LUV channels have no standard deviation.")
std = np.maximum(std, self.min_stds)
self.background_data = ChannelDistributions(
means=mu.tolist(), standard_deviations=std.tolist()
)
class ChannelDeviationLUV(BackgroundDetectAlgorithm):
"""Compare the standard deviations of the LUV channels in a grid to background data.
Using an LUV colour space, each image is divided into an 8x8 grid of images.
The standard deviation of each channel of each image is calculated and compared
to the median standard deviation for a grid of background images.
"""
# Note we don't use the means in this algorithm but we use the same channel
# distributions model
background_data_model: BaseModel = ChannelDistributions
settings_data_model: BaseModel = ColourChannelDetectSettings
# Empirically, 0.5 seems to be approximate the standard deviation for a good image
# in L and V. U appears to be about 60% of this value. U is about 65% of V when
# converting white-noise in RGB into LUV (note that we are using cv2's internal
# LUV colour space not converting to the CIELUV numbers)
min_stds = [0.5, 0.3, 0.5]
def get_sample_coverage(self, image: np.ndarray) -> float:
"""Return the percentage of the input image that is background.
Evaluate whether it is foreground or background by comparing the standard
deviations of an 8x8 grid of sub-images to the median standard deviation
from a background image.
:returns: A value (between 0 and 100) that is the percentage of the image that is
sample.
"""
if not self.background_data:
raise MissingBackgroundDataError(
"Background is not set: you need to calibrate background detection."
)
image_luv = cv2.cvtColor(image, cv2.COLOR_RGB2LUV)
stds = _chunked_stds(image_luv, 8, 8)
bg_stds = self.background_data.standard_deviations
l_cut = bg_stds[0] * self.settings.channel_tolerance
u_cut = bg_stds[1] * self.settings.channel_tolerance
v_cut = bg_stds[2] * self.settings.channel_tolerance
populated_regions = (
(stds[:, :, 0] > l_cut) | (stds[:, :, 1] > u_cut) | (stds[:, :, 2] > v_cut)
)
return float(100 * np.sum(populated_regions) / 64)
def image_is_sample(self, image: np.ndarray) -> tuple[bool, str]:
"""Label the current image as either background or sample.
:returns: A tuple of the result (boolean), and explanation string. The
explanation string is formatted so it can be added into a sentence such as
``An action was taken because the image is {message}.``
"""
sample_coverage = self.get_sample_coverage(image)
# Use bool otherwise get numpy variants of True and False.
is_sample = bool(sample_coverage > self.settings.min_sample_coverage)
message = f"{sample_coverage:0.1f}% sample"
if not is_sample:
message = "only " + message
return is_sample, message
def set_background(self, image: np.ndarray) -> None:
"""Use the input image to update the background distributions."""
image_luv = cv2.cvtColor(image, cv2.COLOR_RGB2LUV)
mu = np.zeros(3)
c_stds = _chunked_stds(image_luv, 8, 8)
channel_blank = np.all(c_stds == 0, axis=(0, 1))
if np.any(channel_blank):
raise ChannelBlankError("Some LUV channels have no standard devaition.")
std = np.median(c_stds, axis=(0, 1))
std = np.maximum(std, self.min_stds)
self.background_data = ChannelDistributions(
means=mu.tolist(), standard_deviations=std.tolist()
)
def _chunked_stds(img: np.ndarray, n_rows: int = 8, n_cols: int = 8) -> np.ndarray:
"""Split image into a grid and calculate std of each channel in each chunk.
:param img: The image to analyse
:param n_rows: The number of rows in the grid
:param n_cols: The number of cols in the grid
:return: A numpy array of the grid of standard deviations.
"""
h, w = img.shape[:2]
row_height = h // n_rows
col_width = w // n_cols
out = np.zeros((n_rows, n_cols, 3))
for i in range(n_rows):
for j in range(n_cols):
chunk = img[
i * row_height : (i + 1) * row_height,
j * col_width : (j + 1) * col_width,
]
out[i, j, :] = np.std(chunk, axis=(0, 1))
return out