import os import pickle import numpy as np from scipy import interpolate from matplotlib import pyplot as plt from matplotlib.path import Path as MatPath from matplotlib.patches import PathPatch from matplotlib.figure import Figure from openflexure_microscope_server import scan_planners THIS_DIR = os.path.dirname(os.path.realpath(__file__)) class FakeSample: """ A fake sample to test scan algorithms. The sample is able to return whether a given position is sample, no image associated with the sample """ def __init__(self, xy_points: list[tuple[int, int]]): """ Create the sample from a spline interpolation around the given points. """ self._sample_perimeter = interp_closed_path(xy_points, 500) def is_sample(self, pos: tuple[int, int], im_size: tuple[int, int]) -> bool: """ Return whether an image at a given location with a given image size is on the sample This doesn't check the entire image feild as this is designed to be used where the fake sample is much larger than the image and has smooth edges It just checks the 4 corners """ img_corners = [ (pos[0] + im_size[0], pos[1] + im_size[1]), (pos[0] + im_size[0], pos[1] - im_size[1]), (pos[0] - im_size[0], pos[1] + im_size[1]), (pos[0] - im_size[0], pos[1] - im_size[1]), ] return any( self._sample_perimeter.contains_point(corner) for corner in img_corners ) @property def patch(self) -> PathPatch: """ The sample as a matplotlib patch fro plotting """ patch = PathPatch(self._sample_perimeter) patch.set(color=(1.0, 0.8, 1.0, 1.0)) return patch def visualise_scan(sample: FakeSample, planner: scan_planners.ScanPlanner) -> Figure: """ For a given sample and scanner object return a matplotlib figure of the scan """ fig, ax = plt.subplots(figsize=(8, 8)) ax.add_artist(sample.patch) xh, yh = zip(*planner._path_history) xi, yi, _ = zip(*planner._imaged_locations) # convert history to numpy array so can calculate quiver arrows xh = np.array(xh) yh = np.array(yh) plt.quiver( xh[:-1], yh[:-1], xh[1:] - xh[:-1], yh[1:] - yh[:-1], scale_units="xy", angles="xy", scale=1, ) plt.plot(xh, yh, "r.") plt.plot(xi, yi, "g*") ax.axis("equal") return fig def interp_closed_path(xy_points: list[tuple[int, int]], n_points: int) -> MatPath: """ Given a lists of xy_points interpolate an n_point closed curve. This can be used to creat an arbitrary sample shape plan a scan. Modified from: https://stackoverflow.com/questions/33962717/interpolating-a-closed-curve-using-scipy """ # Use zip to seperate x and y points into tuples x, y = zip(*xy_points) # Append first point and convert to array x = np.array(x + (x[0],)) y = np.array(y + (y[0],)) # fit splines to x=f(u) and y=g(u), treating both as periodic. also note that s=0 # is needed in order to force the spline fit to pass through all the input points. spline_data, _ = interpolate.splprep([x, y], s=0, per=True) # evaluate the spline xi, yi = interpolate.splev(np.linspace(0, 1, n_points), spline_data) # Convert to a matplotlib closed path path_points = [[xp, yp] for xp, yp in (zip(xi, yi))] return MatPath(path_points, closed=True) def example_smart_spiral() -> tuple[FakeSample, scan_planners.ScanPlanner]: """ Run an example scan and return the sample scanned and the planner object after scan is complete """ xy_sample_points = [ (-5000, -5000), (-2000, 10000), (1000, 2000), (6000, 7000), (9000, 2000), ] sample = FakeSample(xy_sample_points) img_size = (1000, 1000) intial_position = (0, 0) planner_settings = {"dx": 700, "dy": 700, "max_dist": 100000} planner = scan_planners.SmartSpiral( intial_position=intial_position, planner_settings=planner_settings ) while not planner.scan_complete: xy_pos, _ = planner.get_next_location_and_z_estimate() xyz_pos = (xy_pos[0], xy_pos[1], 0) imaged = sample.is_sample(xy_pos, img_size) planner.mark_location_visited(xyz_pos, imaged=imaged, focused=imaged) return sample, planner def profile_and_save_plot_for_example_smart_spiral(): """ Run the example scan and save a plot and the profile data Also print the cumulative stats This runs if you run this file directly """ import pstats import cProfile profiler = cProfile.Profile() sample, planner = profiler.runcall(example_smart_spiral) stats_fname = os.path.join(THIS_DIR, "scan_example_stats.pstats") profiler.dump_stats(stats_fname) png_fname = os.path.join(THIS_DIR, "scan_example_plot.png") fig = visualise_scan(sample, planner) fig.savefig(png_fname, dpi=200) run_stats = pstats.Stats(stats_fname) run_stats.strip_dirs() run_stats.sort_stats("cumulative") run_stats.print_stats("scan_planners.py") def update_example_smart_spiral_pickle(): """ Pickle the ScanPlanner for the example_smart_spiral(), this is done so the history can be compared by testing to check the algorithm is unchanged. If the algorithm is purposefully changed then this will need to be run to update the pickle for the test to pass. """ pkl_fname = os.path.join(THIS_DIR, "example_smart_spiral.pkl") with open(pkl_fname, "wb") as pkl_file_obj: _, planner = example_smart_spiral() pickle.dump(planner, pkl_file_obj, pickle.HIGHEST_PROTOCOL) def get_expected_result_for_example_smart_spiral(): """ Return the expected ScanPlanner object for the example_smart_spiral(), this is pickled, so that it can be committed. """ pkl_fname = os.path.join(THIS_DIR, "example_smart_spiral.pkl") with open(pkl_fname, "rb") as pkl_file_obj: planner = pickle.load(pkl_file_obj) return planner if __name__ == "__main__": profile_and_save_plot_for_example_smart_spiral()