Looping autofocus errors on failure to find focus. Test looping autofocus algorithm
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2 changed files with 118 additions and 12 deletions
97
tests/test_autofocus.py
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97
tests/test_autofocus.py
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"""Tests for the autofoucs logic.
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This doesn't check the behaviour of the JPEG shaprness monitor.
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"""
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import pytest
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import numpy as np
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from openflexure_microscope_server.things.autofocus import (
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AutofocusThing,
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NoFocusFoundError,
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)
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def fake_sharpeness_data(
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dz: int, start_z: int, max_loc: int, length: int = 41
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) -> tuple[list[float], np.ndarray, np.ndarray]:
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"""Create some fake data for the shapeness.
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The highest returned sharpness is closest to max_loc
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"""
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# Some fake timestamps
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times = [i / 10 + 100000 for i in range(length)]
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img_dz = dz / (length - 1)
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heights = [round(start_z + i * img_dz) for i in range(length)]
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# Shapeness is falls off linearly in this model.
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sharpnesses = [10 * dz - abs(max_loc - h) for h in heights]
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return times, np.array(heights), np.array(sharpnesses)
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@pytest.mark.parametrize(
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("start_z", "max_loc", "centre", "attempts_expected", "passes"),
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[
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# To complete the max must be in the central 1200, so -600 to 600 when looping
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# from -1000 to 1000
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(0, 550, True, 1, True), # Found in loop1 from -1000 to 1000
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(0, 650, True, 2, True), # Just outside the limit in loop1
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(0, 1300, True, 2, True), # Found in loop2 from 0 to 2000
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(0, 1300, False, 1, True), # Found in loop1 from 0 to 2000 (as start="base")
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(0, -1300, True, 2, True), # Found in loop2 from 0 to 2000
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(0, 7300, True, 8, True), # Found in loop8 from 6000 to 8000
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(0, 9300, True, 10, True), # Found in loop10 from 8000 to 10000
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(0, 9900, True, 10, False), # Still not central in loop 10, doesn't pass
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],
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)
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def test_looping_autofocus(start_z, max_loc, centre, attempts_expected, passes, mocker):
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"""Test the high level looping autofocus algorithm."""
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dz = 2000
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# Make a mock stage where move_absolute abs and relative updates the position counter.
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stage = mocker.Mock()
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stage.position = {"x": 0, "y": 0, "z": start_z}
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def set_pos(**kwargs: int) -> None:
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"""Move absolute should update position. So make a side effect for the mock."""
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for axis, value in kwargs.items():
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stage.position[axis] = value
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def adjust_pos(**kwargs: int) -> None:
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"""Move relative should update position. So make a side effect for the mock."""
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for axis, value in kwargs.items():
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stage.position[axis] += value
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stage.move_absolute.side_effect = set_pos
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stage.move_relative.side_effect = adjust_pos
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# Make a mock sharpness monitor that can generate sharpness data.
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sharpness_monitor = mocker.MagicMock()
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sharpness_monitor.focus_rel.return_value = (0, 0)
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def return_shapness(*_args) -> tuple[list[float], np.ndarray, np.ndarray]:
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"""Generate shapenesses based on parameterised input, and mock stage position."""
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return fake_sharpeness_data(
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dz=dz,
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start_z=stage.position["z"],
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max_loc=max_loc,
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)
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sharpness_monitor.move_data.side_effect = return_shapness
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autofocus_thing = AutofocusThing()
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if passes:
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autofocus_thing.looping_autofocus(
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stage=stage,
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sharpness_monitor=sharpness_monitor,
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dz=dz,
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start="centre" if centre else "base",
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)
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else:
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with pytest.raises(NoFocusFoundError):
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autofocus_thing.looping_autofocus(
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stage=stage,
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sharpness_monitor=sharpness_monitor,
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dz=dz,
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start="centre" if centre else "base",
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)
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assert sharpness_monitor.focus_rel.call_count == attempts_expected
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assert abs(max_loc - stage.position["z"]) < dz / 40
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