Complete testing of HistoScanWorkflow
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2 changed files with 153 additions and 6 deletions
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@ -422,20 +422,22 @@ class HistoScanWorkflow(ScanWorkflow[HistoScanSettingsModel]):
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:return: A tuple of whether an image was taken, and the z-position for focus.
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If failed to find focus, returns for the focus z-position.
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"""
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# If skipping background, take an image to check if current field of view is background
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if settings.skip_background:
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# If skipping background, take an image to check if current field of view
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# is background
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image_array = self._cam.grab_as_array(stream_name="lores")
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capture_image, bg_message = self._background_detector.image_is_sample(
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image_array
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)
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del image_array
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if not capture_image:
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msg = f"Skipping {xyz_pos} as it is {bg_message}."
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self.logger.info(msg)
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return False, None
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if not capture_image:
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# Return early if the image is background.
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msg = f"Skipping {xyz_pos} as it is {bg_message}."
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self.logger.info(msg)
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return False, None
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save_on_failure = settings.skip_background
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save_on_failure = not settings.skip_background
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focus_height: Optional[int]
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focused, focus_height = self._autofocus.run_smart_stack(
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@ -445,6 +447,10 @@ class HistoScanWorkflow(ScanWorkflow[HistoScanSettingsModel]):
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# An image was captured if we are focussed or we are not skipping background.
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imaged = focused or save_on_failure
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if not imaged:
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msg = f"Stack failed at {xyz_pos} treating as background."
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self.logger.info(msg)
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# run_smart_stage always returns a focus height for the sharpest image even
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# if it failed to find a good focus. Set to None if not focussed.
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if not focused:
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@ -9,6 +9,7 @@ from pydantic import BaseModel
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import labthings_fastapi as lt
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from labthings_fastapi.testing import create_thing_without_server
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from openflexure_microscope_server.scan_planners import SmartSpiral
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from openflexure_microscope_server.stitching import StitchingSettings
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from openflexure_microscope_server.things.autofocus import SmartStackParams
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from openflexure_microscope_server.things.camera_stage_mapping import csm_img_to_stage
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@ -190,6 +191,146 @@ def test_histo_calculate_overlap(csm_matrix, overlap, expected_steps, histo_work
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assert (dx, dy) == expected_steps
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def test_histo_pre_scan_routine(histo_workflow, mocker):
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"""Check the pre-scan routine does autofocusses."""
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# Rather than create a whole Setting class, just create a mock with the value
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# we need set
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mock_settings = mocker.Mock()
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mock_settings.smart_stack_params.autofocus_dz = 1234
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# Run the function
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histo_workflow.pre_scan_routine(mock_settings)
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# Check the autofocus was run using the mocked slot.
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assert histo_workflow._autofocus.looping_autofocus.call_count == 1
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call_kwargs = histo_workflow._autofocus.looping_autofocus.call_args.kwargs
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assert call_kwargs["dz"] == 1234
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assert call_kwargs["start"] == "centre"
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def test_histo_new_scan_planner(histo_workflow, mocker):
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"""Check the pre-scan routine does autofocusses."""
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# Rather than create a whole Setting class, just create a mock with the 3 values
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# we need set
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mock_settings = mocker.Mock()
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mock_settings.dx = 666
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mock_settings.dy = 999
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mock_settings.max_dist = 54321
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planner = histo_workflow.new_scan_planner(
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mock_settings, position={"x": 1, "y": 2, "z": 3}
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)
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assert isinstance(planner, SmartSpiral)
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# Check the settings were read
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assert planner._dx == 666
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assert planner._dy == 999
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assert planner._max_dist == 54321
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def test_histo_acquisition_with_background_detected(histo_workflow, mocker, caplog):
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"""If background is detected it should return without a stack."""
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# Mocking for settings as above
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mock_settings = mocker.Mock()
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mock_settings.skip_background = True
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histo_workflow._background_detector.image_is_sample.return_value = (
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False,
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"totally empty",
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)
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with caplog.at_level(logging.INFO):
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imaged, focus_height = histo_workflow.acquisition_routine(
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mock_settings, xyz_pos=(1, 2, 3)
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)
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# Check returns
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assert imaged is False
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assert focus_height is None # None meaning not focussed
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# Check there is a log explaining what happened
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assert len(caplog.records) == 1
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assert caplog.records[0].message == "Skipping (1, 2, 3) as it is totally empty."
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assert caplog.records[0].levelname == "INFO"
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# And that smart stack never ran
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assert histo_workflow._autofocus.run_smart_stack.call_count == 0
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def test_histo_acquisition_not_skipping_background(histo_workflow, mocker, caplog):
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"""Check when not skipping background an image is always saved."""
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# Mocking for settings as above
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mock_settings = mocker.Mock()
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mock_settings.skip_background = False
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# Set up background detector to say it is empty, this shouldn't stop stacking.
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histo_workflow._background_detector.image_is_sample.return_value = (
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False,
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"totally empty",
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)
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with caplog.at_level(logging.INFO):
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# First set smart stack to report failure
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histo_workflow._autofocus.run_smart_stack.return_value = (False, 123)
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imaged, focus_height = histo_workflow.acquisition_routine(
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mock_settings, xyz_pos=(1, 2, 3)
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)
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# Check return
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assert imaged is True # Always images if not skipping background
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assert focus_height is None # None meaning didn't focus
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# And again reporting a successful stack
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histo_workflow._autofocus.run_smart_stack.return_value = (True, 123)
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imaged, focus_height = histo_workflow.acquisition_routine(
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mock_settings, xyz_pos=(1, 2, 3)
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)
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# Check return
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assert imaged is True # Always images if not skipping background
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assert focus_height == 123
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# Should never warn
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assert len(caplog.records) == 0
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# And that smart stack never ran
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assert histo_workflow._autofocus.run_smart_stack.call_count == 2
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def test_histo_acquisition_on_sample(histo_workflow, mocker, caplog):
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"""Check when skipping background, but background not detected."""
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# Mocking for settings as above
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mock_settings = mocker.Mock()
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mock_settings.skip_background = True
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# Set up background detector to say there is sample.
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histo_workflow._background_detector.image_is_sample.return_value = (True, None)
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with caplog.at_level(logging.INFO):
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# First set smart stack to report failure
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histo_workflow._autofocus.run_smart_stack.return_value = (False, 123)
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imaged, focus_height = histo_workflow.acquisition_routine(
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mock_settings, xyz_pos=(1, 2, 3)
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)
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# Check return:
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# Don't save failed stack when skipping background, assume it is actually
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# background
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assert imaged is False
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assert focus_height is None # None meaning didn't focus
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# And again reporting a successful stack
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histo_workflow._autofocus.run_smart_stack.return_value = (True, 123)
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imaged, focus_height = histo_workflow.acquisition_routine(
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mock_settings, xyz_pos=(1, 2, 3)
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)
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# Check return
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assert imaged is True # Always images if not skipping background
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assert focus_height == 123
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# Should never warn
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assert len(caplog.records) == 1
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expected_message = "Stack failed at (1, 2, 3) treating as background."
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assert caplog.records[0].message == expected_message
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assert caplog.records[0].levelname == "INFO"
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# And that smart stack never ran
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assert histo_workflow._autofocus.run_smart_stack.call_count == 2
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def test_histo_workflow_settings_ui(histo_workflow):
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"""Check that the workflow specifies the expected controls."""
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ui = histo_workflow.settings_ui
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