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Merge branch 'stack-height-sanitise' into 'v3'

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Verify stack params at start of scan main loop

Closes #455

See merge request openflexure/openflexure-microscope-server!400
This commit is contained in:
Julian Stirling 2025-10-09 14:00:59 +00:00
commit 94541b2d4f
4 changed files with 244 additions and 68 deletions
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184
src/openflexure_microscope_server/things/autofocus.py
View file

@ -16,7 +16,7 @@ from dataclasses import dataclass
from fastapi import Depends
import numpy as np
from pydantic import BaseModel
from pydantic import BaseModel, field_validator, computed_field, model_validator
import labthings_fastapi as lt
from labthings_fastapi.types.numpy import NDArray
@ -26,54 +26,23 @@ from .camera import CameraDependency as CameraClient
from .stage import StageDependency as Stage
LOGGER = logging.getLogger(__name__)
MIN_TEST_IMAGE_COUNT = 3
MAX_TEST_IMAGE_COUNT = 9
class NotStreamingError(RuntimeError):
"""No images captured from stream. The camera is almost certainly not streaming."""
class StackParams:
class StackParams(BaseModel):
"""A class for holding for stack parameters, and returning computed ones."""
def __init__(
self,
*,
stack_dz: int,
images_to_save: int,
min_images_to_test: int,
autofocus_dz: int,
images_dir: str,
save_resolution: tuple[int, int],
) -> None:
"""Initialise the parameters. All arguments are keyword only.
:param stack_dz: The number of motor steps between images
:param images_to_save: The number of images to save to disk
:param min_images_to_test: The minimum number of images in the stack before,
the stack is evaluated for focus. As more images are captured evaluation
of the focus is always evaluated with the same number of images. i.e. if
``min_images_to_test=9``, then 9 images are captured, if the stack is not
well focused, a 10th image is captured and images 2 to 10 are evaluated
for focus
:param autofocus_dz: The number of steps in a full autofocus (when required)
:param images_dir: The directory to save images to disk
:param save_resolution: The resolution to save the captures to disk with
"""
if min_images_to_test < images_to_save:
raise ValueError("Can't save more images than the minimum number tested")
if min_images_to_test % 2 == 0 or min_images_to_test <= 0:
raise ValueError(
"Minimum number of images to test should be positive and odd"
)
if images_to_save % 2 == 0 or images_to_save <= 0:
raise ValueError("Images to save must be positive and odd")
self.stack_dz = stack_dz
self.images_to_save = images_to_save
self.min_images_to_test = min_images_to_test
self.autofocus_dz = autofocus_dz
self.images_dir = images_dir
self.save_resolution = save_resolution
stack_dz: int
images_to_save: int
min_images_to_test: int
autofocus_dz: int
images_dir: str
save_resolution: tuple[int, int]
# Using docstrings under variables as this is how pdoc would expect
# attributed to be documented
@ -102,6 +71,41 @@ class StackParams:
max_attempts: int = 3
"""Maximum number of times to attempt fast stack"""
@field_validator("min_images_to_test")
@classmethod
def check_images_to_test(cls, min_images_to_test: int) -> int:
"""Verify that the images to test parameter matches various constraints."""
if min_images_to_test < MIN_TEST_IMAGE_COUNT:
raise ValueError(
f"Can't test for focus with fewer than {MIN_TEST_IMAGE_COUNT} images."
)
if min_images_to_test > MAX_TEST_IMAGE_COUNT:
raise ValueError(
f"Testing with more than {MAX_TEST_IMAGE_COUNT} images is likely to focus on the cover slip, or strike the sample."
)
if min_images_to_test % 2 == 0 or min_images_to_test <= 0:
raise ValueError(
"Minimum number of images to test should be positive and odd"
)
return min_images_to_test
@field_validator("images_to_save")
@classmethod
def check_images_to_save(cls, images_to_save: int) -> int:
"""Verify that the images to save parameter is positive and odd."""
if images_to_save % 2 == 0 or images_to_save <= 0:
raise ValueError("Images to save must be positive and odd")
return images_to_save
@model_validator(mode="after")
def check_image_limits(self) -> "StackParams":
"""Ensure the number of images to save isn't more than the minimum tested."""
if self.images_to_save > self.min_images_to_test:
raise ValueError("Can't save more images than the minimum number tested.")
return self
# Note MyPy doesn't support decorating properties. See MyPy Pull #16571 and issue #14461.
@computed_field # type: ignore[prop-decorator]
@property
def stack_z_range(self) -> int:
"""The range of the z stack, in steps.
@ -112,6 +116,7 @@ class StackParams:
"""
return self.stack_dz * (self.min_images_to_test - 1)
@computed_field # type: ignore[prop-decorator]
@property
def steps_undershoot(self) -> int:
"""The distance to deliberately undershoot the estimated optimal starting point."""
@ -121,6 +126,7 @@ class StackParams:
# requires extra +z movements and captures.
return self.stack_dz * self.img_undershoot
@computed_field # type: ignore[prop-decorator]
@property
def max_images_to_test(self) -> int:
"""The maximum number of images that will be captured and tested in a stack.
@ -467,7 +473,7 @@ class AutofocusThing(lt.Thing):
enough more images may be captured. After new images are captured the number sets
the number of images used for checking if focus is central.
Defaults to 9 which balances reliability and speed/
Defaults to 9 which balances reliability and speed.
"""
stack_dz = lt.ThingSetting(initial_value=50, model=int)
@ -480,15 +486,86 @@ class AutofocusThing(lt.Thing):
* 200 for 20x
"""
@lt.thing_action
def create_stack_params(
self,
images_dir: str,
autofocus_dz: int,
save_resolution: tuple[int, int],
logger: lt.deps.InvocationLogger,
) -> StackParams:
"""Set up the parameters used for all stacks in a scan.
:param images_dir: the folder to save all images
:param autofocus_dz: the range to autofocus over if a stack fails
:param save_resolution: The resolution to save the captures to disk with
:param logger: A logger passed from the calling Thing
:returns: A StackParams object with the required parameters.
"""
# Coerce min_images_to_test parameter
min_images_to_test = self.stack_min_images_to_test
if min_images_to_test < MIN_TEST_IMAGE_COUNT:
logger.warning(
f"Cannot test only {min_images_to_test} image(s) as this will fail. "
"Setting min images to test to lowest possible value of"
f"{MIN_TEST_IMAGE_COUNT}."
)
min_images_to_test = MIN_TEST_IMAGE_COUNT
elif min_images_to_test > MAX_TEST_IMAGE_COUNT:
logger.warning(
f"Testing {min_images_to_test} images will cause defocus. "
"Setting min images to test to highest possible value of "
f"{MAX_TEST_IMAGE_COUNT}."
)
min_images_to_test = MAX_TEST_IMAGE_COUNT
elif min_images_to_test % 2 == 0:
min_images_to_test += 1
logger.warning(
"Minimum number of images to test should be odd, setting to "
f"{min_images_to_test}."
)
# Set the Thing property to the coerced value
self.stack_min_images_to_test = min_images_to_test
# Coerce the images to save parameter to be positive, odd, and less than
# min_images_to_save
images_to_save = self.stack_images_to_save
if images_to_save <= 0:
logger.warning(
"At least 1 images must be saved. Setting images to save to 1."
)
images_to_save = 1
elif images_to_save > min_images_to_test:
logger.warning(
f"Cannot save {images_to_save} images as this above the minimum "
f"number to test. Setting images to save to {MAX_TEST_IMAGE_COUNT}."
)
images_to_save = min_images_to_test
elif images_to_save % 2 == 0:
images_to_save += 1
logger.warning(
f"Images to save should be odd, setting to {images_to_save}."
)
# Set the Thing property to the coerced value
self.stack_images_to_save = images_to_save
return StackParams(
stack_dz=self.stack_dz,
images_to_save=self.stack_images_to_save,
min_images_to_test=self.stack_min_images_to_test,
autofocus_dz=autofocus_dz,
images_dir=images_dir,
save_resolution=save_resolution,
)
@lt.thing_action
def run_smart_stack(
self,
cam: CameraClient,
stage: Stage,
sharpness_monitor: SharpnessMonitorDep,
images_dir: str,
autofocus_dz: int,
save_resolution: tuple[int, int],
stack_parameters: StackParams,
) -> tuple[bool, int]:
"""Run a smart stack.
@ -503,27 +580,14 @@ class AutofocusThing(lt.Thing):
:param stage: Stage Dependency supplied by LabThings dependency injection
:param sharpness_monitor: Sharpness Monitor Dependency (for focus detection)
supplied by LabThings dependency injection
:param images_dir: the folder to save all images
:param autofocus_dz: the range to autofocus over if a stack fails
:param save_resolution: The resolution the images should be saved at, the
images will be resampled if this doesn't match the camera's capture
resolution
:param stack_parameters: A StackParams object containing the required
parameters to run a stack.
:returns: A tuple containing:
* A boolean, True if stack was successfully
* The z position of the sharpest image
"""
# Set the variables to prevent changes from the GUI or other windows
stack_parameters = StackParams(
stack_dz=self.stack_dz,
images_to_save=self.stack_images_to_save,
min_images_to_test=self.stack_min_images_to_test,
autofocus_dz=autofocus_dz,
images_dir=images_dir,
save_resolution=save_resolution,
)
tries = 0
# Loop until a stack is successful
while tries < stack_parameters.max_attempts:

15
src/openflexure_microscope_server/things/smart_scan.py
View file

@ -26,7 +26,7 @@ from openflexure_microscope_server import scan_planners
from openflexure_microscope_server import stitching
# Things
from .autofocus import AutofocusThing
from .autofocus import AutofocusThing, StackParams
from .camera_stage_mapping import CameraStageMapper
from .camera import CameraDependency as CameraClient
from .stage import StageDependency as StageDep
@ -121,7 +121,7 @@ class SmartScanThing(lt.Thing):
self._stage: Optional[StageDep] = None
self._cam: Optional[CameraClient] = None
self._csm: Optional[CSMDep] = None
self._stack_params: Optional[StackParams] = None
self._ongoing_scan: Optional[scan_directories.ScanDirectory] = None
self._scan_data: Optional[scan_directories.ScanData] = None
self._preview_stitcher: Optional[stitching.PreviewStitcher] = None
@ -189,6 +189,7 @@ class SmartScanThing(lt.Thing):
self._scan_lock.release()
# Ensure any PreviewStitcher created cannot be reused.
self._preview_stitcher = None
self._stack_params = None
# Remove any scan folders containing zero images.
self.purge_empty_scans(logger=logger)
@ -363,6 +364,12 @@ class SmartScanThing(lt.Thing):
self._cam.start_streaming(main_resolution=(3280, 2464))
self._scan_data = self._collect_scan_data()
self._ongoing_scan.save_scan_data(self._scan_data)
self._stack_params = self._autofocus.create_stack_params(
images_dir=self._ongoing_scan.images_dir,
autofocus_dz=self.autofocus_dz,
save_resolution=self._scan_data.save_resolution,
logger=self._scan_logger,
)
self._preview_stitcher = stitching.PreviewStitcher(
self._ongoing_scan.images_dir,
overlap=self._scan_data.overlap,
@ -451,9 +458,7 @@ class SmartScanThing(lt.Thing):
continue
focused, focused_height = self._autofocus.run_smart_stack(
images_dir=self._ongoing_scan.images_dir,
autofocus_dz=self._scan_data.autofocus_dz,
save_resolution=self._scan_data.save_resolution,
stack_parameters=self._stack_params
)
current_pos_xyz = (new_pos_xyz[0], new_pos_xyz[1], focused_height)