Fully subclass Rectangle basescanner for raster and snake

This commit is contained in:
jaknapper 2026-02-09 19:35:19 +00:00
parent fb7a8b4959
commit 6294cde471
3 changed files with 156 additions and 12 deletions

View file

@ -19,6 +19,7 @@
},
"histo_scan_workflow": "openflexure_microscope_server.things.scan_workflows:HistoScanWorkflow",
"snake_workflow": "openflexure_microscope_server.things.scan_workflows:SnakeWorkflow",
"raster_workflow": "openflexure_microscope_server.things.scan_workflows:RasterWorkflow",
"stage_measure": "openflexure_microscope_server.things.stage_measure:RangeofMotionThing",
"bg_color_channels_luv": "openflexure_microscope_server.things.background_detect:ColourChannelDetectLUV",
"bg_channel_deviations_luv": "openflexure_microscope_server.things.background_detect:ChannelDeviationLUV"

View file

@ -433,7 +433,7 @@ class SmartSpiral(RectangleScan):
def _parse(self, planner_settings: Optional[dict] = None) -> None:
super()._parse(planner_settings)
if "max_dist" not in planner_settings:
if not planner_settings or "max_dist" not in planner_settings:
raise KeyError("SmartSpiral requires max_dist")
self._max_dist = int(planner_settings["max_dist"])
@ -614,7 +614,9 @@ class SnakeScan(RectangleScan):
expected_keys = ["x_count", "y_count"]
invalid_msg = "SnakeScan requires planner_settings with keys: "
if not all(k in planner_settings for k in expected_keys):
if not planner_settings or not all(
k in planner_settings for k in expected_keys
):
raise KeyError(invalid_msg + ",".join(expected_keys))
self._x_count = int(planner_settings["x_count"])
@ -654,7 +656,9 @@ class RasterScan(RectangleScan):
expected_keys = ["x_count", "y_count"]
invalid_msg = "SnakeScan requires planner_settings with keys: "
if not all(k in planner_settings for k in expected_keys):
if not planner_settings or not all(
k in planner_settings for k in expected_keys
):
raise KeyError(invalid_msg + ",".join(expected_keys))
self._x_count = int(planner_settings["x_count"])

View file

@ -519,7 +519,7 @@ class SnakeSettingsModel(BaseModel):
save_resolution: tuple[int, int]
class SnakeWorkflow(ScanWorkflow[SettingModelType], Generic[SettingModelType]):
class SnakeWorkflow(ScanWorkflow[SnakeSettingsModel]):
"""A workflow optimised for snaking around samples.
This workflow generates a list of coordinates in a rectangle, and snakes
@ -535,7 +535,7 @@ class SnakeWorkflow(ScanWorkflow[SettingModelType], Generic[SettingModelType]):
readonly=True,
)
_settings_model: type[SettingModelType] = SnakeSettingsModel
_settings_model = SnakeSettingsModel
_planner_cls: type[ScanPlanner] = SnakeScan
# Thing Slots
_cam: BaseCamera = lt.thing_slot()
@ -578,7 +578,7 @@ class SnakeWorkflow(ScanWorkflow[SettingModelType], Generic[SettingModelType]):
def all_settings(
self, images_dir: str
) -> tuple[SettingModelType, StitchingSettings]:
) -> tuple[SnakeSettingsModel, StitchingSettings]:
"""Return the workflow and stitching settings.
:param images_dir: The directory that images are to be written to.
@ -609,7 +609,7 @@ class SnakeWorkflow(ScanWorkflow[SettingModelType], Generic[SettingModelType]):
return scan_settings, stitching_settings
def pre_scan_routine(self, settings: SettingModelType) -> None:
def pre_scan_routine(self, settings: SnakeSettingsModel) -> None:
"""Autofocus before starting the scan.
:param settings: The settings for this scan as a SnakeSettingsModel
@ -617,7 +617,7 @@ class SnakeWorkflow(ScanWorkflow[SettingModelType], Generic[SettingModelType]):
self._autofocus.looping_autofocus(dz=settings.autofocus_dz, start="centre")
def new_scan_planner(
self, settings: SettingModelType, position: Mapping[str, int]
self, settings: SnakeSettingsModel, position: Mapping[str, int]
) -> ScanPlanner:
"""Return a new scan planner object.
@ -640,7 +640,7 @@ class SnakeWorkflow(ScanWorkflow[SettingModelType], Generic[SettingModelType]):
)
def acquisition_routine(
self, settings: SettingModelType, xyz_pos: tuple[int, int, int]
self, settings: SnakeSettingsModel, xyz_pos: tuple[int, int, int]
) -> tuple[bool, Optional[int]]:
"""Perform acquisition routine. This is run at each scan location.
@ -671,16 +671,23 @@ class SnakeWorkflow(ScanWorkflow[SettingModelType], Generic[SettingModelType]):
]
class RasterSettingsModel(SnakeSettingsModel):
class RasterSettingsModel(BaseModel):
"""The settings for a scan with the Raster Workflow.
This is identical to the SnakeSettings, but is subclassed for clarity.
"""
pass
overlap: float
dx: int
dy: int
x_count: int
y_count: int
images_dir: str
autofocus_dz: int
save_resolution: tuple[int, int]
class RasterWorkflow(SnakeWorkflow[RasterSettingsModel]):
class RasterWorkflow(ScanWorkflow[RasterSettingsModel]):
"""A workflow optimised for snaking around samples.
This workflow generates a list of coordinates in a rectangle, and always
@ -699,3 +706,135 @@ class RasterWorkflow(SnakeWorkflow[RasterSettingsModel]):
_settings_model = RasterSettingsModel
_planner_cls: type[ScanPlanner] = RasterScan
# Thing Slots
_cam: BaseCamera = lt.thing_slot()
_csm: CameraStageMapper = lt.thing_slot()
_autofocus: AutofocusThing = lt.thing_slot()
_stage: BaseStage = lt.thing_slot()
# Scan settings
autofocus_dz: int = lt.setting(default=1000, ge=200, le=2000)
"""The z distance to perform an autofocus in steps.
Must be greater than or equal to 200, and less than or equal to 2000.
"""
overlap: float = lt.setting(default=0.45, ge=0.1, le=0.7)
"""The fraction that adjacent images should overlap in x or y.
This must be between 0.1 and 0.7.
"""
x_count: int = lt.setting(default=3)
y_count: int = lt.setting(default=2)
# The noqa statement is because scan_name is unused but is needed for equivalence
# with other workflows that may want to validate the scan name.
def check_before_start(self, scan_name: str) -> None: # noqa: ARG002
"""Before starting a scan, check that camera-stage-mapping is set.
Raise error if:
- camera stage mapping is not set
"""
if self._csm.calibration_required:
raise RuntimeError("Camera Stage Mapping is not calibrated.")
@lt.property
def ready(self) -> bool:
"""Whether this scanworkflow is ready to start."""
return not self._csm.calibration_required
def all_settings(
self, images_dir: str
) -> tuple[RasterSettingsModel, StitchingSettings]:
"""Return the workflow and stitching settings.
:param images_dir: The directory that images are to be written to.
:return: A tuple containing the settings model for this workflow and the
settings model for stitching.
"""
stitching_settings = StitchingSettings(
overlap=self.overlap,
correlation_resize=STITCHING_RESOLUTION[0] / self.save_resolution[0],
)
dx, dy = self._calc_displacement_from_overlap(self.overlap)
self.logger.info(
f"Based on an overlap of {self.overlap}, the stage will make steps of "
f"{dx}, {dy}"
)
scan_settings = self._settings_model(
overlap=self.overlap,
dx=dx,
dy=dy,
x_count=self.x_count,
y_count=self.y_count,
images_dir=images_dir,
autofocus_dz=self.autofocus_dz,
save_resolution=self.save_resolution,
)
return scan_settings, stitching_settings
def pre_scan_routine(self, settings: RasterSettingsModel) -> None:
"""Autofocus before starting the scan.
:param settings: The settings for this scan as a SnakeSettingsModel
"""
self._autofocus.looping_autofocus(dz=settings.autofocus_dz, start="centre")
def new_scan_planner(
self, settings: RasterSettingsModel, position: Mapping[str, int]
) -> ScanPlanner:
"""Return a new scan planner object.
:param settings: The settings for this scan as a SnakeSettingsModel
:param position: The starting position as a mapping of axes names to int.
"""
# The initial plan for the scan should be a single x,y position. All future
# moves will be planned around this point. In future, route planner could
# have multiple starting positions, each of which will be visited before the
# scan can end.
planner_settings = {
"dx": settings.dx,
"dy": settings.dy,
"x_count": settings.x_count,
"y_count": settings.y_count,
}
return self._planner_cls(
initial_position=(position["x"], position["y"]),
planner_settings=planner_settings,
)
def acquisition_routine(
self, settings: RasterSettingsModel, xyz_pos: tuple[int, int, int]
) -> tuple[bool, Optional[int]]:
"""Perform acquisition routine. This is run at each scan location.
:param settings: The settings for this scan as a RasterSettingsModel
:param xyz_pos: The current position as a tuple or 3 ints.
:return: A tuple of whether an image was taken, and the z-position for focus.
If failed to find focus, returns for the focus z-position.
"""
self._autofocus.fast_autofocus(dz=settings.autofocus_dz)
focus_height = self._stage.get_xyz_position()[2]
filename = f"img_{xyz_pos[0]}_{xyz_pos[1]}_{focus_height}.jpeg"
self._cam.capture_and_save(
jpeg_path=os.path.join(settings.images_dir, filename),
save_resolution=settings.save_resolution,
)
imaged = True
return imaged, focus_height
@lt.property
def settings_ui(self) -> list[PropertyControl]:
"""A list of PropertyControl objects to create the settings in the scan tab."""
return [
property_control_for(self, "overlap", label="Image Overlap (0.1-0.7)"),
property_control_for(self, "x_count", label="Number of columns"),
property_control_for(self, "y_count", label="Number of rows"),
property_control_for(self, "autofocus_dz", label="Autofocus Range (steps)"),
]