Create aquisition routines for scan workflows, remove background detector from base ScanWorkflow class

This commit is contained in:
Julian Stirling 2026-01-15 14:53:47 +00:00
parent b6343362b2
commit 54ed78c832
2 changed files with 86 additions and 52 deletions

View file

@ -7,7 +7,6 @@ from openflexure_microscope_server.scan_planners import ScanPlanner, SmartSpiral
from openflexure_microscope_server.stitching import STITCHING_RESOLUTION
from openflexure_microscope_server.things.autofocus import AutofocusThing
from openflexure_microscope_server.things.background_detect import (
BackgroundDetectAlgorithm,
ChannelDeviationLUV,
)
from openflexure_microscope_server.things.camera_stage_mapping import CameraStageMapper
@ -20,14 +19,10 @@ class ScanWorkflow(lt.Thing):
scan planning, aquisition routine.
"""
# All workdlows must have at least one background detector and a set class for scan
# planning
_background_detector: Optional[
BackgroundDetectAlgorithm | Mapping[str, BackgroundDetectAlgorithm]
] = lt.thing_slot()
# All workdlows must have a set class for scan planning
_planner_cls: type[ScanPlanner]
# All workdlows set a save resolution
# All workflows set a save resolution
save_resolution: tuple[int, int] = lt.setting(default=(1640, 1232))
"""A tuple of the image resolution to capture."""
@ -56,15 +51,26 @@ class ScanWorkflow(lt.Thing):
is returned if it is not possible to stitch the scan.
"""
raise NotImplementedError(
"Each specific ScanWorkflow must implement a `all_settings`. "
"method."
"Each specific ScanWorkflow must implement a `all_settings`. method."
)
def pre_scan_routine(self) -> None:
def pre_scan_routine(self, settings: dict) -> None:
raise NotImplementedError(
"Each specific ScanWorkflow must implement a pre-scan routine."
)
def new_scan_planner(self, settings: dict, position: Mapping[str, int]) -> None:
raise NotImplementedError(
"Each specific ScanWorkflow must implement a ``new_scan_planner`` method."
)
def aquisition_routine(
self, settings: dict, xyz_pos: tuple[int, int, int]
) -> tuple[bool, Optional[int]]:
raise NotImplementedError(
"Each specific ScanWorkflow must implement an aquisition routine"
)
class HistoScanWorkflow(ScanWorkflow):
# Thing Slots
@ -184,5 +190,53 @@ class HistoScanWorkflow(ScanWorkflow):
# If not use the other stage axes
return x_move_stage["y"], y_move_stage["x"]
def pre_scan_routine(self) -> None:
self._autofocus.looping_autofocus(dz=self.autofocus_dz, start="centre")
def pre_scan_routine(self, settings: dict) -> None:
self._autofocus.looping_autofocus(dz=settings["autofocus_dz"], start="centre")
def new_scan_planner(self, settings: dict, position: Mapping[str, int]) -> None:
# The initial plan for the scan should be a single x,y position. All future
# moves will be planned around this point. In future, route planner could
# have multiple starting positions, each of which will be visited before the
# scan can end.
planner_settings = {
"dx": settings["dx"],
"dy": settings["dy"],
"max_dist": settings["max_dist"],
}
return self._planner_cls(
initial_position=(position["x"], position["y"]),
planner_settings=planner_settings,
)
def aquisition_routine(
self, settings: dict, xyz_pos: tuple[int, int, int]
) -> tuple[bool, Optional[int]]:
"""Perform aquisition routine. This is run at each scan location.
:return: A tuple of whether an image was taken, and the z-position for focus.
If failed to find focus, returns for the focus z-position.
"""
# If skipping background, take an image to check if current field of view is background
if settings["skip_background"]:
capture_image, bg_message = self._cam.image_is_sample()
if not capture_image:
msg = f"Skipping {xyz_pos} as it is {bg_message}."
self.logger.info(msg)
return False, None
save_on_failure = settings["skip_background"]
focused, focused_height = self._autofocus.run_smart_stack(
stack_parameters=self.stack_params,
save_on_failure=save_on_failure,
)
# An image was captured if we are focussed or we are not skipping background.
imaged = focused or save_on_failure
# run_smart_stage always returns a focus height for the sharpest image even
# if it failed to find a good focus. Set to None if not focussed.
if not focused:
focused_height = None
return imaged, focused_height

View file

@ -22,19 +22,18 @@ from typing import (
TypeVar,
)
import numpy as np
from fastapi import HTTPException
from fastapi.responses import FileResponse
from pydantic import BaseModel
import labthings_fastapi as lt
from openflexure_microscope_server import scan_directories, scan_planners, stitching
from openflexure_microscope_server import scan_directories, stitching
# Things
from .autofocus import AutofocusThing, StackParams
from .camera import BaseCamera
from .camera_stage_mapping import CameraStageMapper, CSMUncalibratedError
from .camera_stage_mapping import CameraStageMapper
from .scan_workflows import HistoScanWorkflow, ScanWorkflow
from .stage import BaseStage
@ -284,6 +283,9 @@ class SmartScanThing(lt.Thing):
@_scan_running
def _manage_stitching_threads(self) -> None:
"""Manage the stitching threads, starting them if needed and not already running."""
if self.scan_data.stitching_settings is None:
# This scan can't stitch.
return
# Assume 4 images means at least one offset in x and y, making the stitching
# well constrained.
if self.scan_data.image_count > 3 and not self.preview_stitcher.running:
@ -354,18 +356,9 @@ class SmartScanThing(lt.Thing):
This loop runs during a scan, until no more scan x,y positions
are remaining.
"""
# The initial plan for the scan should be a single x,y position. All future
# moves will be planned around this point. In future, route planner could
# have multiple starting positions, each of which will be visited before the
# scan can end.
planner_settings = {
"dx": self.scan_data.dx,
"dy": self.scan_data.dy,
"max_dist": self.scan_data.max_dist,
}
route_planner = scan_planners.SmartSpiral(
initial_position=(self._stage.position["x"], self._stage.position["y"]),
planner_settings=planner_settings,
workflow_settings = self.scan_data.workflow_settings
route_planner = workflow.new_scan_planner(
workflow_settings, self._stage.position
)
# The loop tests if the scan should continue, moves to the next position,
@ -383,43 +376,30 @@ class SmartScanThing(lt.Thing):
new_pos_xyz[1],
self._stage.position["z"],
)
capture_image = True
# If skipping background, take an image to check if current field of view is background
if self.scan_data.skip_background:
capture_image, bg_message = self._cam.image_is_sample()
if not capture_image:
route_planner.mark_location_visited(
new_pos_xyz, imaged=False, focused=False
)
msg = f"Skipping {new_pos_xyz} as it is {bg_message}."
self.logger.info(msg)
continue
focused, focused_height = self._autofocus.run_smart_stack(
stack_parameters=self.stack_params,
save_on_failure=not self.scan_data.skip_background,
imaged, focus_height = workflow.aquisition_routine(
workflow_settings, current_pos_xyz
)
current_pos_xyz = (new_pos_xyz[0], new_pos_xyz[1], focused_height)
# An image was captured if we are focussed or we are not skipping background.
imaged = focused or not self.scan_data.skip_background
if focus_height is None:
focused = False
else:
current_pos_xyz = (new_pos_xyz[0], new_pos_xyz[1], focus_height)
route_planner.mark_location_visited(
current_pos_xyz, imaged=imaged, focused=focused
)
# increment capture counter as thread has completed
self.scan_data.image_count += 1
# Add it to the incremental zip
self.ongoing_scan.zip_files()
if imaged:
# increment capture counter as thread has completed
self.scan_data.image_count += 1
# Add it to the incremental zip
self.ongoing_scan.zip_files()
@_scan_running
def _return_to_starting_position(self) -> None:
"""Return to the initial scan position, if set."""
self.logger.info("Returning to starting position.")
if self._scan_data is not None:
self._stage.move_absolute(
**self.scan_data.starting_position, block_cancellation=True