Separate "classic" fast AF from up-down-up AF
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e6913b23e2
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4ceb6bcafe
1 changed files with 72 additions and 54 deletions
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@ -218,18 +218,14 @@ def move_and_measure(microscope, dz):
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return m.data_dict()
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return m.data_dict()
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def fast_autofocus(microscope, dz=2000, backlash=None):
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def fast_autofocus(microscope, dz=2000):
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"""Perform a down-up-down-up autofocus"""
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"""Perform a down-up-down-up autofocus"""
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with monitor_sharpness(
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with microscope.camera.lock, microscope.stage.lock:
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microscope
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with monitor_sharpness(microscope) as m:
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) as m, microscope.camera.lock as _, microscope.stage.lock as _:
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i, z = m.focus_rel(-dz / 2)
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i, z = m.focus_rel(-dz / 2)
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i, z = m.focus_rel(dz)
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i, z = m.focus_rel(dz)
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fz = m.sharpest_z_on_move(i)
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fz = m.sharpest_z_on_move(i)
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if backlash is None:
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i, z = m.focus_rel(-dz) # move all the way to the start so it's consistent
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i, z = m.focus_rel(-dz) # move all the way to the start so it's consistent
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else:
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i, z = m.focus_rel(fz - z - backlash)
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m.focus_rel(fz - z)
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m.focus_rel(fz - z)
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return m.data_dict()
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return m.data_dict()
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@ -272,9 +268,8 @@ def fast_up_down_up_autofocus(
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might slightly hurt accuracy, but is unlikely to be a big issue. Too big
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might slightly hurt accuracy, but is unlikely to be a big issue. Too big
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may cause you to overshoot, which is a problem.
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may cause you to overshoot, which is a problem.
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"""
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"""
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with monitor_sharpness(
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with microscope.camera.lock, microscope.stage.lock:
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microscope
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with monitor_sharpness(microscope) as m:
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) as m, microscope.camera.lock as _, microscope.stage.lock as _:
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# Ensure the MJPEG stream has started
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# Ensure the MJPEG stream has started
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microscope.camera.start_stream_recording()
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microscope.camera.start_stream_recording()
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@ -346,7 +341,6 @@ class MoveAndMeasureAPI(ActionView):
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if microscope.has_real_stage():
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if microscope.has_real_stage():
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# Acquire microscope lock with 1s timeout
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# Acquire microscope lock with 1s timeout
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with microscope.lock(timeout=1):
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with microscope.lock(timeout=1):
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# Run fast_up_down_up_autofocus
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return move_and_measure(microscope, dz=args.get("dz"))
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return move_and_measure(microscope, dz=args.get("dz"))
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else:
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else:
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@ -384,6 +378,30 @@ class FastAutofocusAPI(ActionView):
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Run a fast autofocus
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Run a fast autofocus
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"""
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"""
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args = {"dz": fields.Int(missing=2000)}
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def post(self, args):
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microscope = find_component("org.openflexure.microscope")
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if not microscope:
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abort(503, "No microscope connected. Unable to autofocus.")
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if microscope.has_real_stage():
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logging.debug("Running autofocus...")
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# Acquire microscope lock with 1s timeout
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with microscope.lock(timeout=1):
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# Run fast_autofocus
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return fast_autofocus(microscope, dz=args.get("dz"))
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else:
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abort(503, "No stage connected. Unable to autofocus.")
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class UpDownUpAutofocusAPI(ActionView):
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"""
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Run a fast up-down-up autofocus
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"""
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args = {
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args = {
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"dz": fields.Int(missing=2000),
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"dz": fields.Int(missing=2000),
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"backlash": fields.Int(missing=25, minimum=0),
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"backlash": fields.Int(missing=25, minimum=0),
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