Went on a PEP-8 rampage
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36 changed files with 186 additions and 218 deletions
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@ -3,21 +3,21 @@ import numpy as np
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from openflexure_microscope.api.v1.views import MicroscopeViewPlugin
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from openflexure_microscope.api.utilities import JsonPayload
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from flask import request, Response, escape, jsonify
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from flask import request, jsonify
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import logging
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class MeasureSharpnessAPI(MicroscopeViewPlugin):
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def post(self):
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payload = JsonPayload(request)
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return jsonify({'sharpness': self.plugin.measure_sharpness()})
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class AutofocusAPI(MicroscopeViewPlugin):
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def post(self):
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payload = JsonPayload(request)
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# Figure out the range of z values to use
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dz = payload.param("dz", default=np.linspace(-300,300,7), convert=np.array)
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dz = payload.param("dz", default=np.linspace(-300, 300, 7), convert=np.array)
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print("Running autofocus...")
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task = self.microscope.task.start(self.plugin.autofocus, dz)
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@ -1,21 +1,24 @@
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import numpy as np
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from scipy import ndimage
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def decimate_to(shape, image):
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"""Decimate an image to reduce its size if it's too big."""
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decimation = np.max(np.ceil(np.array(image.shape, dtype=np.float)[:len(shape)]/np.array(shape)))
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return image[::int(decimation), ::int(decimation), ...]
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def sharpness_sum_lap2(rgb_image):
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"""Return an image sharpness metric: sum(laplacian(image)**")"""
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#image_bw=np.mean(decimate_to((1000,1000), rgb_image),2)
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image_bw=np.mean(rgb_image, 2)
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image_lap=ndimage.filters.laplace(image_bw)
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# image_bw=np.mean(decimate_to((1000,1000), rgb_image),2)
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image_bw = np.mean(rgb_image, 2)
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image_lap = ndimage.filters.laplace(image_bw)
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return np.mean(image_lap.astype(np.float)**4)
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def sharpness_edge(image):
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"""Return a sharpness metric optimised for vertical lines"""
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gray = np.mean(image.astype(float), 2)
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n = 20
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edge = np.array([[-1]*n + [1]*n])
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return np.sum([np.sum(ndimage.filters.convolve(gray,W)**2) for W in [edge, edge.T]])
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return np.sum([np.sum(ndimage.filters.convolve(gray, W)**2) for W in [edge, edge.T]])
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@ -34,7 +34,7 @@ class AutofocusPlugin(MicroscopePlugin):
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positions = []
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camera.annotate_text = ""
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for i in stage.scan_z(dz, return_to_start=False):
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for _ in stage.scan_z(dz, return_to_start=False):
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positions.append(stage.position[2])
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time.sleep(settle)
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sharpnesses.append(self.measure_sharpness(metric_fn))
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@ -46,4 +46,4 @@ class AutofocusPlugin(MicroscopePlugin):
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def measure_sharpness(self, metric_fn=sharpness_sum_lap2):
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"""Measure the sharpness of the camera's current view."""
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return metric_fn(self.microscope.camera.array(use_video_port=True, resize=(640,480)))
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return metric_fn(self.microscope.camera.array(use_video_port=True, resize=(640, 480)))
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@ -1,28 +1,26 @@
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import time
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import numpy as np
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from openflexure_microscope.plugins import MicroscopePlugin
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from openflexure_microscope.utilities import set_properties
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from openflexure_microscope.api.v1.views import MicroscopeViewPlugin
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from openflexure_microscope.api.utilities import JsonPayload
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from flask import request, Response, escape, jsonify
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from flask import request, jsonify
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import logging
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from .recalibrate_utils import recalibrate_camera
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class RecalibrateAPIView(MicroscopeViewPlugin):
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def post(self):
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payload = JsonPayload(request)
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# Figure out the range of z values to use
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# TODO: Figure out the range of z values to use
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print("Starting microscope recalibration...")
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task = self.microscope.task.start(self.plugin.recalibrate)
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# return a handle on the autofocus task
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# Return a handle on the autofocus task
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return jsonify(task.state, 202)
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class Plugin(MicroscopePlugin):
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"""
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A set of default plugins
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@ -45,16 +43,14 @@ class Plugin(MicroscopePlugin):
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streaming = scamera.state['stream_active']
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if streaming:
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logging.info("Stopping stream before recalibration")
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scamera.stop_stream_recording(resolution=(640,480))
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scamera.stop_stream_recording(resolution=(640, 480))
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old_resolution = scamera.camera.resolution
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try:
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scamera.camera.resolution=(640,480)
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scamera.camera.resolution = (640, 480)
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recalibrate_camera(scamera.camera)
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finally:
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scamera.camera.resolution=old_resolution
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scamera.camera.resolution = old_resolution
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self.microscope.save_config()
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if streaming:
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logging.info("Restarting stream after recalibration")
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scamera.start_stream_recording()
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@ -3,12 +3,14 @@ from picamera import PiCamera
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from picamera.array import PiRGBArray, PiBayerArray
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import time
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def rgb_image(camera, resize=None, **kwargs):
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"""Capture an image and return an RGB numpy array"""
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with PiRGBArray(camera, size=resize) as output:
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camera.capture(output, format='rgb', resize=resize, **kwargs)
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return output.array
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def flat_lens_shading_table(camera):
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"""Return a flat (i.e. unity gain) lens shading table.
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@ -20,6 +22,7 @@ def flat_lens_shading_table(camera):
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raise ImportError("This program requires the forked picamera library with lens shading support")
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return np.zeros(camera._lens_shading_table_shape(), dtype=np.uint8) + 32
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def adjust_exposure_to_setpoint(camera, setpoint):
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"""Adjust the camera's exposure time until the maximum pixel value is <setpoint>."""
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print("Adjusting shutter speed to hit setpoint {}".format(setpoint), end="")
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@ -29,11 +32,12 @@ def adjust_exposure_to_setpoint(camera, setpoint):
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time.sleep(1)
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print("done")
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def auto_expose_and_freeze_settings(camera):
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"""Freeze the settings after auto-exposing to white illumination"""
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print("Allowing the camera to auto-expose")
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camera.awb_mode="auto"
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camera.exposure_mode="auto"
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camera.awb_mode = "auto"
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camera.exposure_mode = "auto"
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for i in range(6):
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print(".", end="")
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time.sleep(0.5)
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@ -54,18 +58,19 @@ def auto_expose_and_freeze_settings(camera):
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def channels_from_bayer_array(bayer_array):
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"""Given the 'array' from a PiBayerArray, return the 4 channels."""
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bayer_pattern = [(i//2, i%2) for i in range(4)]
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bayer_pattern = [(i//2, i % 2) for i in range(4)]
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channels = np.zeros((4, bayer_array.shape[0]//2, bayer_array.shape[1]//2), dtype=bayer_array.dtype)
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for i, offset in enumerate(bayer_pattern):
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# We simplify life by dealing with only one channel at a time.
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channels[i, :, :] = np.sum(bayer_array[offset[0]::2, offset[1]::2, :], axis=2)
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channels[i, :, :] = np.sum(bayer_array[offset[0]::2, offset[1]::2, :], axis=2)
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return channels
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def lst_from_channels(channels):
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"""Given the 4 Bayer colour channels from a white image, generate a LST."""
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full_resolution = np.array(channels.shape[1:]) * 2 # channels have been binned
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#lst_resolution = list(np.ceil(full_resolution / 64.0).astype(int))
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full_resolution = np.array(channels.shape[1:]) * 2 # channels have been binned
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# lst_resolution = list(np.ceil(full_resolution / 64.0).astype(int))
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lst_resolution = [(r // 64) + 1 for r in full_resolution]
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# NB the size of the LST is 1/64th of the image, but rounded UP.
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print("Generating a lens shading table at {}x{}".format(*lst_resolution))
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@ -73,7 +78,7 @@ def lst_from_channels(channels):
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for i in range(lens_shading.shape[0]):
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image_channel = channels[i, :, :]
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iw, ih = image_channel.shape
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ls_channel = lens_shading[i,:,:]
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ls_channel = lens_shading[i, :, :]
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lw, lh = ls_channel.shape
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# The lens shading table is rounded **up** in size to 1/64th of the size of
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# the image. Rather than handle edge images separately, I'm just going to
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@ -84,19 +89,23 @@ def lst_from_channels(channels):
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# less computationally efficient!
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padded_image_channel = np.pad(image_channel,
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[(0, lw*32 - iw), (0, lh*32 - ih)],
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mode="edge") # Pad image to the right and bottom
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print("Channel shape: {}x{}, shading table shape: {}x{}, after padding {}".format(iw,ih,lw*32,lh*32,padded_image_channel.shape))
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mode="edge") # Pad image to the right and bottom
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print("Channel shape: {}x{}, shading table shape: {}x{}, after padding {}".format(iw,
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ih,
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lw*32,
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lh*32,
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padded_image_channel.shape))
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# Next, fill the shading table (except edge pixels). Please excuse the
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# for loop - I know it's not fast but this code needn't be!
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box = 3 # We average together a square of this side length for each pixel.
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box = 3 # We average together a square of this side length for each pixel.
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# NB this isn't quite what 6by9's program does - it averages 3 pixels
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# horizontally, but not vertically.
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for dx in np.arange(box) - box//2:
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for dy in np.arange(box) - box//2:
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ls_channel[:,:] += padded_image_channel[16+dx::32,16+dy::32] - 64
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ls_channel[:, :] += padded_image_channel[16+dx::32, 16+dy::32] - 64
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ls_channel /= box**2
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# The original C code written by 6by9 normalises to the central 64 pixels in each channel.
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#ls_channel /= np.mean(image_channel[iw//2-4:iw//2+4, ih//2-4:ih//2+4])
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# ls_channel /= np.mean(image_channel[iw//2-4:iw//2+4, ih//2-4:ih//2+4])
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# I have had better results just normalising to the maximum:
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ls_channel /= np.max(ls_channel)
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# NB the central pixel should now be *approximately* 1.0 (may not be exactly
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@ -107,11 +116,12 @@ def lst_from_channels(channels):
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# What we actually want to calculate is the gains needed to compensate for the
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# lens shading - that's 1/lens_shading_table_float as we currently have it.
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gains = 32.0/lens_shading # 32 is unity gain
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gains[gains > 255] = 255 # clip at 255, maximum gain is 255/32
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gains[gains < 32] = 32 # clip at 32, minimum gain is 1 (is this necessary?)
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gains = 32.0/lens_shading # 32 is unity gain
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gains[gains > 255] = 255 # clip at 255, maximum gain is 255/32
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gains[gains < 32] = 32 # clip at 32, minimum gain is 1 (is this necessary?)
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lens_shading_table = gains.astype(np.uint8)
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return lens_shading_table[::-1,:,:].copy()
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return lens_shading_table[::-1, :, :].copy()
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def recalibrate_camera(camera):
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"""Reset the lens shading table and exposure settings.
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@ -124,7 +134,7 @@ def recalibrate_camera(camera):
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``StreamingCamera``.
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"""
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camera.lens_shading_table = flat_lens_shading_table(camera)
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discarded = rgb_image(camera) # for some reason the camera won't work unless I do this!
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_ = rgb_image(camera) # for some reason the camera won't work unless I do this!
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with PiBayerArray(camera) as a:
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camera.capture(a, format="jpeg", bayer=True)
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@ -132,18 +142,19 @@ def recalibrate_camera(camera):
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# Now we need to calculate a lens shading table that would make this flat.
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# raw_image is a 3D array, with full resolution and 3 colour channels. No
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# demosaicing has been done, so 2/3 of the values are zero (3/4 for R and B
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# channels, 1/2 for green because there's twize as many green pixels).
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# de-mosaicing has been done, so 2/3 of the values are zero (3/4 for R and B
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# channels, 1/2 for green because there's twice as many green pixels).
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channels = channels_from_bayer_array(raw_image)
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lens_shading_table = lst_from_channels(channels)
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camera.lens_shading_table=lens_shading_table
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test = rgb_image(camera)
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camera.lens_shading_table = lens_shading_table
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_ = rgb_image(camera)
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# Fix the AWB gains so the image is neutral
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channel_means = np.mean(np.mean(rgb_image(camera), axis=0, dtype=np.float), axis=0)
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old_gains = camera.awb_gains
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camera.awb_gains = (channel_means[1]/channel_means[0] * old_gains[0], channel_means[1]/channel_means[2]*old_gains[1])
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camera.awb_gains = (channel_means[1]/channel_means[0] * old_gains[0],
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channel_means[1]/channel_means[2]*old_gains[1])
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time.sleep(1)
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# Ensure the background is bright but not saturated
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adjust_exposure_to_setpoint(camera, 230)
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@ -157,4 +168,3 @@ if __name__ == "__main__":
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recalibrate_camera(camera)
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print("Done.")
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time.sleep(2)
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