Use backlash compensation in smart scan and autofocus
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parent
c12814f0eb
commit
0527990282
4 changed files with 21 additions and 13 deletions
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@ -22,7 +22,7 @@ import labthings_fastapi as lt
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from labthings_fastapi.types.numpy import NDArray
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from .camera import BaseCamera, CaptureParams
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from .stage import BaseStage
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from .stage import BacklashCompensation, BaseStage
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LOGGER = logging.getLogger(__name__)
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MIN_TEST_IMAGE_COUNT = 3
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@ -458,14 +458,17 @@ class AutofocusThing(lt.Thing):
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up to 10 times.
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"""
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attempt = 0
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backlash = 200
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with JPEGSharpnessMonitor(self._stage, self._cam) as sharpness_monitor:
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while attempt < 10:
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attempt += 1
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if start == "centre":
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self._stage.move_relative(x=0, y=0, z=-int(backlash + dz / 2))
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self._stage.move_relative(x=0, y=0, z=backlash)
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self._stage.move_relative(
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x=0,
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y=0,
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z=-int(dz / 2),
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backlash_compensation=BacklashCompensation.Z_ONLY,
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)
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# Always start centrally for future runs
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start = "centre"
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@ -480,8 +483,10 @@ class AutofocusThing(lt.Thing):
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target_max = np.max(heights) - dz / 5
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# move to the peak
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self._stage.move_absolute(z=peak_height - backlash)
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self._stage.move_absolute(z=peak_height)
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self._stage.move_absolute(
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z=peak_height,
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backlash_compensation=BacklashCompensation.Z_ONLY,
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)
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if target_min < peak_height < target_max:
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# If it is within the target range then return
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@ -622,12 +627,10 @@ class AutofocusThing(lt.Thing):
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# Better to start too low and take too many images than too high and need to refocus
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self._stage.move_relative(
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z=-int(
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stack_parameters.steps_undershoot
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+ stack_parameters.backlash_correction
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+ stack_parameters.stack_z_range / 2
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)
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stack_parameters.steps_undershoot + stack_parameters.stack_z_range / 2
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),
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backlash_compensation=BacklashCompensation.Z_ONLY,
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)
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self._stage.move_relative(z=stack_parameters.backlash_correction)
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captures: list[CaptureInfo] = []
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# Always check for focus using the the last `min_images_to_test` in the
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@ -37,7 +37,7 @@ from openflexure_microscope_server.utilities import coerce_thing_selector
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# Things
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from .camera import BaseCamera
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from .scan_workflows import ScanWorkflow
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from .stage import BaseStage
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from .stage import BacklashCompensation, BaseStage
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T = TypeVar("T")
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P = ParamSpec("P")
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@ -311,6 +311,7 @@ class SmartScanThing(lt.Thing):
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x=next_point[0],
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y=next_point[1],
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z=z_estimate,
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backlash_compensation=BacklashCompensation.XY_ONLY,
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)
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return (next_point[0], next_point[1], z_estimate)
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@ -142,7 +142,9 @@ class BaseStage(lt.Thing):
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"""Current position of the stage."""
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return self._apply_axis_direction(self._hardware_position)
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backlash_steps: dict[str, int] = lt.setting(default={"x": 200, "y": 200, "z": 200})
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backlash_steps: dict[str, int] = lt.setting(
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default={"x": 200, "y": 200, "z": 200}, readonly=True
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)
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"""The number of steps to elimate backlash. The sign sets the direction.
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A positive number sets the direction of the second move in a backlash correction.
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@ -60,6 +60,8 @@ def test_looping_autofocus(start_z, max_loc, centre, attempts_expected, passes,
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def adjust_pos(**kwargs: int) -> None:
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"""Move relative should update position. So make a side effect for the mock."""
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# Remove backlash compensation from the dict if it exists.
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kwargs.pop("backlash_compensation", None)
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for axis, value in kwargs.items():
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autofocus_thing._stage.position[axis] += value
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