Add raster scan, with generic typing to allow subclassed settings
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3 changed files with 97 additions and 13 deletions
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@ -14,6 +14,7 @@
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},
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"histo_scan_workflow": "openflexure_microscope_server.things.scan_workflows:HistoScanWorkflow",
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"snake_workflow": "openflexure_microscope_server.things.scan_workflows:SnakeWorkflow",
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"raster_workflow": "openflexure_microscope_server.things.scan_workflows:RasterWorkflow",
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"bg_color_channels_luv": "openflexure_microscope_server.things.background_detect:ColourChannelDetectLUV",
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"bg_channel_deviations_luv": "openflexure_microscope_server.things.background_detect:ChannelDeviationLUV"
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},
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@ -637,14 +637,67 @@ class SnakeScan(ScanPlanner):
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return self._grid_to_future_locations(grid)
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# The noqa statement is because next_position is unused but is needed for equivalence
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# with other workflows that require the next pos to select a neighbour.
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def select_nearby_focus_site(self, next_position: XYPos) -> Optional[XYZPos]: # noqa: ARG002
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"""For a snake scan, use the most recent focused site to predict focus."""
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def select_nearby_focus_site(self, next_position: XYPos) -> Optional[XYZPos]:
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"""Return a focused site near the given position to estimate Z for the next move.
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Looks for all previously focused locations that are within the scan
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step size (self._dx, self._dy) of `next_position`. Among these nearby focused
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sites, it returns the most recently imaged one.
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This is suitable for raster or snake scans, where the scan may move along a row
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or column and then jump to a new row/column. If no nearby focused sites exist,
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returns None.
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:param next_position: The XY position where the next image will be taken.
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:return: The XYZ tuple of the closest and most recent focused site, or None if
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no focused locations exist.
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"""
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focused_locations = self.focused_locations
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if not focused_locations:
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return None
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return focused_locations[-1]
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next_pos_arr = np.array(next_position, dtype="float64")
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path_arr = np.array(focused_locations, dtype="float64")[:, :2]
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# Find all focused positions within dx and dy of next_position
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dx_ok = np.abs(path_arr[:, 0] - next_pos_arr[0]) <= self._dx
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dy_ok = np.abs(path_arr[:, 1] - next_pos_arr[1]) <= self._dy
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nearby_indices = np.where(dx_ok & dy_ok)[0]
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if len(nearby_indices) == 0:
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return None
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# Pick the most recent nearby site
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return focused_locations[nearby_indices[-1]]
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class RasterScan(SnakeScan):
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"""A scan planner that performs a snake scan, always moving right and down.
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This planner starts at the corner of the region to scan, and always moves right
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to the end of the row, then down to the next row (assuming positive dx, dy).
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This is subclassed from SnakeScan, as the only difference in behaviour is in
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building the initial path.
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"""
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def _initial_location_list(self) -> list[FutureScanLocation]:
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"""Set the initial list of locations for this scan planner.
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This is called on initialisation.
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For raster scan, this is the full grid, and none will be added during scanning.
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"""
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grid = create_rectangular_scan_path(
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starting_pos=self._initial_position,
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x_count=self._x_count,
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y_count=self._y_count,
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dx=self._dx,
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dy=self._dy,
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style="raster",
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)
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return self._grid_to_future_locations(grid)
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def distance_between(
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@ -19,6 +19,7 @@ from pydantic import BaseModel
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import labthings_fastapi as lt
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from openflexure_microscope_server.scan_planners import (
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RasterScan,
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ScanPlanner,
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SmartSpiral,
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SnakeScan,
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@ -518,7 +519,7 @@ class SnakeSettingsModel(BaseModel):
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save_resolution: tuple[int, int]
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class SnakeWorkflow(ScanWorkflow[SnakeSettingsModel]):
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class SnakeWorkflow(ScanWorkflow[SettingModelType], Generic[SettingModelType]):
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"""A workflow optimised for snaking around samples.
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This workflow generates a list of coordinates in a rectangle, and snakes
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@ -534,10 +535,9 @@ class SnakeWorkflow(ScanWorkflow[SnakeSettingsModel]):
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readonly=True,
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)
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_settings_model = SnakeSettingsModel
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_settings_model: type[SettingModelType] = SnakeSettingsModel
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_planner_cls: type[ScanPlanner] = SnakeScan
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# Thing Slots
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_background_detector: ChannelDeviationLUV = lt.thing_slot()
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_cam: BaseCamera = lt.thing_slot()
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_csm: CameraStageMapper = lt.thing_slot()
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_autofocus: AutofocusThing = lt.thing_slot()
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@ -578,7 +578,7 @@ class SnakeWorkflow(ScanWorkflow[SnakeSettingsModel]):
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def all_settings(
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self, images_dir: str
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) -> tuple[SnakeSettingsModel, StitchingSettings]:
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) -> tuple[SettingModelType, StitchingSettings]:
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"""Return the workflow and stitching settings.
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:param images_dir: The directory that images are to be written to.
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@ -596,7 +596,7 @@ class SnakeWorkflow(ScanWorkflow[SnakeSettingsModel]):
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f"{dx}, {dy}"
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)
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scan_settings = SnakeSettingsModel(
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scan_settings = self._settings_model(
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overlap=self.overlap,
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dx=dx,
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dy=dy,
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@ -609,7 +609,7 @@ class SnakeWorkflow(ScanWorkflow[SnakeSettingsModel]):
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return scan_settings, stitching_settings
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def pre_scan_routine(self, settings: SnakeSettingsModel) -> None:
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def pre_scan_routine(self, settings: SettingModelType) -> None:
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"""Autofocus before starting the scan.
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:param settings: The settings for this scan as a SnakeSettingsModel
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@ -617,7 +617,7 @@ class SnakeWorkflow(ScanWorkflow[SnakeSettingsModel]):
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self._autofocus.looping_autofocus(dz=settings.autofocus_dz, start="centre")
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def new_scan_planner(
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self, settings: SnakeSettingsModel, position: Mapping[str, int]
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self, settings: SettingModelType, position: Mapping[str, int]
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) -> ScanPlanner:
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"""Return a new scan planner object.
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@ -640,7 +640,7 @@ class SnakeWorkflow(ScanWorkflow[SnakeSettingsModel]):
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)
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def acquisition_routine(
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self, settings: SnakeSettingsModel, xyz_pos: tuple[int, int, int]
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self, settings: SettingModelType, xyz_pos: tuple[int, int, int]
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) -> tuple[bool, Optional[int]]:
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"""Perform acquisition routine. This is run at each scan location.
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@ -669,3 +669,33 @@ class SnakeWorkflow(ScanWorkflow[SnakeSettingsModel]):
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property_control_for(self, "y_count", label="Number of rows"),
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property_control_for(self, "autofocus_dz", label="Autofocus Range (steps)"),
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]
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class RasterSettingsModel(SnakeSettingsModel):
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"""The settings for a scan with the Raster Workflow.
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This is identical to the SnakeSettings, but is subclassed for clarity.
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"""
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pass
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class RasterWorkflow(SnakeWorkflow[RasterSettingsModel]):
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"""A workflow optimised for snaking around samples.
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This workflow generates a list of coordinates in a rectangle, and always
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moves right across a row, then moves down a row while moving to the starting
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column (assuming positive dx and dy).
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"""
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display_name: str = lt.property(default="Raster Scan", readonly=True)
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ui_blurb: str = lt.property(
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default=(
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"This scan workflow is optimised for performing a raster scan over a rectangle. It "
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"always moves down and right from the starting point, over a defined grid."
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),
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readonly=True,
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)
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_settings_model = RasterSettingsModel
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_planner_cls: type[ScanPlanner] = RasterScan
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